| 产品介绍 | | |
| Mouse anti Bovine GFAP antibody, clone 1B4recognizes glial fibrillary acidic protein, or GFAP, a class III intermediate filament. During development of the central nervous system, GFAP distinguishes astrocytes from other glial cells. It is involved in various cellular functions, such as cell structure and movement, cell communication and the functioning of the blood-brain barrier. It also plays a role in mitosis by adjusting the filament network present in the cell.Defects in GFAP are a cause of Alexander disease, a rare disorder of the central nervous system affecting mostly males. It is a progressive leukoencephalopathy resulting in mental and physical retardation, dementia, seizures and early death.Mouse anti Bovine GFAP antibody, clone 1B4 may be used in conjunction with clones4A11and2E1for increased sensitivity when used in immunohistology. |
| 产品详情 | | |
| Target Species | Bovine |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Immunogen | Bovine spinal cord homogenate. |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/25 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Pegram, C.N.et al.(1985) Monoclonal antibodies reactive with epitopes restricted to glial fibrillary acidic proteins of several species.Neurochem Pathol. 3 (2): 119-38.2.McLendon, R.E.et al.(1986) The immunohistochemical application of three anti-GFAP monoclonal antibodies to formalin-fixed, paraffin-embedded, normal and neoplastic brain tissues.J Neuropathol Exp Neurol. 45 (6): 692-703.3.Schwabenlander, M.et al.(2016) Brain, Craniofacial, and Dental Lesions of a Free-ranging Gray Wolf (Canis lupus) Implicated in a Human Attack in Minnesota, USA.J Wildl Dis. 52 (1): 131-7. |
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