CD4 Monoclonal Antibody (RM4-4), FITC, eBioscience™
货号：11-0043-82 ,11-0043-85
规格：100 μg,500 μg
价格：1112,2630
产品类型：流式抗体
品牌：eBioscience
抗原：CD4
物种：小鼠
宿主：大鼠
抗体亚型：IgG2b, kappa
克隆号：RM4-4
荧光染料：FITC
| 抗体类型: | 流式抗体 | 同型对照： | Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™ |
| 浓度： | 0.5 mg/mL | 用法： | 0.125 µg/test (Flow） |
| 产品详细信息Description: The RM4-4 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by the majority of thymocytes, a subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with the protein tyrosine kinase lck through its cytoplasmic tail. Binding of RM4-4 does not block binding of the CD4 monoclonal antibodies RM4-5 or GK1.5.Applications Reported: This RM4-4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RM4-4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017]. |
| 数据 |
CD4 Antibody (11-0043-81) in FlowC57BL/6 mouse splenocytes were stained with Rat IgG2b kappa Isotype Control, FITC (Product # 11-4031-82) (blue histogram) or CD4 Monoclonal Antibody, FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.CD4 Antibody (11-0043-81)PLoS genetics 2013-Pubilished figure using CD4 monoclonal antibody(Pubilished# 11-0043-81) |
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| 参考文献： |
| 1.Oncoimmunology Contrasting impact of corticosteroids on anti-PD-1 immunotherapy efficacy for tumor histologies located within or outside the central nervous system.2.Experimental and therapeutic medicine Assessment of tumor growth factor-β1 neutralizing antibody in the treatment of allergic rhinitis and asthma.3.BMC immunology Blocking IL-10 signalling at the time of immunization does not increase unwanted side effects in mice.4.Circulation research T-Cell Mineralocorticoid Receptor Controls Blood Pressure by Regulating Interferon-Gamma.5.Nature communications Suppressive IL-17A+Foxp3+ and ex-Th17 IL-17AnegFoxp3+ Treg cells are a source of tumour-associated Treg cells. |
技术参数 产品优点 Flow;IHC;IF;Misc
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