mouse anti Rat MHC CLASS II RT1Bu/l:PE
货号：MCA45PE
规格：100 Tests
价格：3970
产品类型：流式抗体
品牌：Bio-Rad
抗原：MHC Class II RT1Bu/L
物种：大鼠
宿主：小鼠
抗体亚型：IgG1
克隆号：OX-3
荧光染料：PE
| 产品介绍 | | |
| Mouse anti Rat MHC Class II RT1Bu/L antibody, clone OX-3recognizes a polymorphic determinant of the rat RT1B MHC class II antigen, reacting with haplotypes u and l. The literature reports reactivity with Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains. This antibody is useful for distinguishing RT1B positive cells from different rat strains, e.g. for recognising cells of donor origin in bone marrow reconstituted radiation chimaeras.The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H-2 region.Mouse anti Rat MHC Class II RT1Bu/L antibody, clone OX-3 also cross reacts with mouse strains of the H-2 haplotypes b and s. Analysis of recombinant mouse strains has mapped the OX-3 determinant to the H-2I-A region.This product is routinely tested in flow cytometry on Lewis rat splenocytes. |
| 产品详情 | | |
| Target Species | Rat |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Preparation | Purified IgG prepared by ion exchange chromatography from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Immunogen | Rat thymocyte membrane glycoproteins. |
| Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells from the NS1 mouse myeloma cell line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 推荐产品 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:RPE | MCA1209PE | F | 100 Tests |
| 文献 |
| 1.McMaster, W.R. & Williams, A.F. (1979) Identification of Ia glycoproteins in rat thymus and purification from rat spleen.Eur J Immunol. 9 (6): 426-33.2.McMaster, W.R. & Williams, A.F. (1979) Monoclonal antibodies to Ia antigens from rat thymus: cross reactions with mouse and human and use in purification of rat Ia glycoproteins.Immunol Rev. 47: 117-37.3.Barclay, A.N. & Mayrhofer, G. (1981) Bone marrow origin of Ia-positive cells in the medulla rat thymus.J Exp Med. 153 (6): 1666-71.4.Zhang, J.et al.(1997) Expression of major histocompatibility complex molecules in rodent retina. Immunohistochemical study.Invest Ophthalmol Vis Sci. 38 (9): 1848-57.5.ahm, K.B.et al.(2000) Loss of TGF-beta signaling contributes to autoimmune pancreatitis.J Clin Invest. 105 (8): 1057-65. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
推荐用于 Flow
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-31298.htm
QQ:1749072012