| 产品介绍 | | |
| Mouse anti Human C5 antibody, clone 046-10.2.2recognizes secreted complement component 5 (C5), a ~190 kDa serine protease present in blood serum. It is composed of an ~115 kDa disulphide linked alpha-chain and a ~75 kDa beta-chain. In addition to its role in complement, C5 is thought to be associated with inflammation, stimulating tumour necrosis factor alpha, interferon gamma mediated suppression of IL-10 and IL-12 production.C5 is activated via either the classical or the alternative complement pathway, by proteolytic cleavage of C5a from the N-terminal of the alpha-chain, by C5 convertase. C5a is an anaphylotoxin which mediates local inflammation. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. It also stimulates chemotaxis of neutrophils, eosinophils, basophils and monocytes towards the site of inflammation. The remaining fragment, C5b, initiates the assembly of the membrane attack complex.Mouse anti Human C5 antibody, clone 046-10.2.2 blocks C5 haemolytic activity (Harderet al.2017).Removal of sodium azide is recommended prior to use in functional assays. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified Ig - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from ascites |
| Buffer Solution | Borate buffered saline |
| Immunogen | Purified human C5. |
| Approx. Protein Concentrations | Current, batch-specific concentration 1.08 mg/ml |
| Preservative Stabilisers | 0.1% Sodium Azide (NaN3) |
| Regulatory | For research purposes only |
| Guarantee | Guaranteed until date of expiry. Please see product label. |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Karp, C.L.et al.(2000) Identification of complement factor 5 as a susceptibility locus for experimental allergic asthma.Nat Immunol. 1 (3): 221-6.2.Peng, T.et al.(2005) Role of C5 in the development of airway inflammation, airway hyperresponsiveness, and ongoing airway response.J Clin Invest. 115 (6): 1590-600. |
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