| 产品介绍 | | |
| Rat anti Human CD8 antibody, clone YTC182.20recognizes the human CD8 cell surface antigen, a ~30/32 kDa glycoprotein expressed by the cytotoxic/suppressor subset of T lymphocytes, and more weakly by NK cells. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
| Preparation | Antibody purified from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Immunogen | Mouse L cells transfected with human CD8 gene. |
| Approx. Protein Concentrations | IgG concentration 0.1 mg/ml |
| Fusion Partners | Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag.1.2.3 myeloma line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| FITC | 490 | 525 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at +4℃.After reconstitution store at +4℃.DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Jonker, M.et al. (1989) Reactivity of mAb specific for human CD markers with Rhesus monkey leucocytes.Leucocyte Typing IV. Oxford University Press p 1058-1063.2.Yoshino, N.et al.(2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.Exp Anim. 49 (2): 97-110.3.Walton, L.J.et al.(1998) Intra-epithelial subpopulations of T lymphocytes and Langerhans cells in oral lichen planus.J Oral Pathol Med. 27 (3): 116-23.Shacklett, B.L.et al.(2004) Abundant ex |
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