| 产品介绍 | | |
| Mouse anti human NQO1 antibody, clone A180recognizes human NQO1 (NAD(P)H:quinone oxidoreductase 1), a ~31 kDa cytosolic flavoenzyme and member of the NAD(P)H dehydrogenase (quinone) family, involved in detoxification. NQO1 catalyzes the two-electron reduction of quinones to hydroquinones.NQO1 is expressed in a wide range of tissues and is overexpressed in many human tumors including lung, brain, liver, colon and breast. The ability of NQO1 to bioactivate anti-tumor quinones including mitomycin C and diaziquone, has become a focus area for chemotherapeutic investigations. Mutations in the NQO1 gene are associated with an increased risk of certain cancers, and an increased risk of leukaemia has been associated with diminished NQO1 activity and the NQO1*2 allele (Nerbertet al.2002). Mouse anti human NQO1 antibody, clone A180 recognizes the product of the wild-type allele NQO1*1 but does not recognize the product of the mutant NQO1*2 allele (Satoet al.2010andFegerholmet al.2008).Mouse anti human NQO1 antibody, clone A180 detects endogenous levels of total NQO1, and does not cross-react with NQO2 (Siegalet al.1998).Mouse anti human NQO1 antibody, clone A180 is reported as suitable for use in immunofluorescence (Siegalet al.2012). |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Fusion Partners | Spleen cells from immunised rats were fused with cells of the mouse P3-X63-Ag8.56 myeloma cell line. |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Immunogen | Purified recombinant human NQO1 |
| Acknowledgements | PrecisionAb is a trademark of Bio-Rad Laboratories |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Western Blotting | √ | 1/100 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Siegel, D.et al.(1998) Immunohistochemical detection of NAD(P)H:quinone oxidoreductase in human lung and lung tumors.Clin Cancer Res. 4 (9): 2065-70.2.Anwar, A.et al.(2002) Interaction of the molecular chaperone Hsp70 with human NAD(P)H:quinone oxidoreductase 1.J Biol Chem. 277 (16): 14060-7.3.Anwar, A.et al.(2003) Interaction of human NAD(P)H:quinone oxidoreductase 1 (NQO1) with the tumor suppressor protein p53 in cells and cell-free systems.J Biol Chem. 278: 10368-73.4.Moran, J.L.et al.(1999) A potential mechanism underlying the increased susceptibility of individuals with a polymorphism in NAD(P)H:quinone oxidoreductase 1 (NQO1) to benzene toxicity.Proc Natl Acad Sci U S A. 96: 8150-5.5.Zhou, H.et al.(2010) NAD(P)H:quinone oxidoreductase 1-compromised human bone marrow endothelial cells exhibit decreased adhesion molecule expression and CD34+ hematopoietic cell adhesion.J Pharmacol Exp Ther. 334: 260-8. |
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