| 产品介绍 | | |
| Rat anti Mouse CD199 antibody, clone 9B1detects mouse chemokine receptor 9 (CCR9), also known as CD199. CCR9 is a seven transmembrane protein which is a member of the beta chemokines receptor family. CCR9 is expressed in thymus and small intestine, and mediates the recruitment and development of thymocytes in response to CCL25; the only known ligand of CCR9.CCR9 is an alternative co-receptor with CD4 for HIV infection, and has been implicated in adult T cell leukaemia. Two alternatively spliced transcript variants have been described.Clone 9B1 blocks CCL25-induced Ca2+release, attenuating inflammation in a murine model of chronic ileitis when given in early disease stages. |
| 产品详情 | | |
| Target Species | Mouse |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | 25 amino acids from the amino terminal of murine CCR9, coupled to KLH. |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
| Preservative Stabilisers | 0.09%Sodium Azide |
| Fusion Partners | Spleen cells from immunised Lewis rat were fused with cells of the X63-Ag8.653 myeloma cell line. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/25 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Rivera-nieves, J.et al.(2006) Antibody blockade of CCL25/CCR9 ameliorates early but not late chronic murine ileitis.Gastroenterology. 131 (5): 1518-29.2.Nagakubo, D.et al.(2007) Expression of CCR9 in HTLV-1+ T cells and ATL cells expressing Tax.Int J Cancer. 120 (7): 1591-7. |
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