| 产品介绍 | | |
| Mouse anti Human CD2 antibody, clone LT2recognizes human CD2, a single chain transmembrane glycoprotein of 50 kDa, expressed by T cells, NK cells and thymocytes.CD2 is a receptor for CD58 (LFA-3), and is important in mediating the interaction between T cells and target cells or antigen presenting cells.Mouse anti Human CD2 antibody, clone LT2 inhibits NK cell mediated cytotoxicity, and ConA and PHA mediated blast transformation. |
| 产品详情 | | |
Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Rhesus Monkey | √ |
| Chimpanzee | √ |
| Cynomolgus monkey | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG conjugated to Alexa Fluor® 647 - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide 1% Bovine Serum Albumin 0.1% Pluronic F68 0.1% PEG 33500.05% Tween 20 |
Immunogen | Normal human peripheral blood granulocytes. |
Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| Max Ex/Em | 340/513 |
| 存储条件 | | |
| Store at +4°C. DO NOT FREEZE.This product should be stored undiluted. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 5ul of the suggested working dilution to label 106cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
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| 相关产品 |
Description | Product Code | Applications | Pack Size |
Human Seroblock | BUF070A | F | 50 Test |
Human Seroblock | BUF070B | F | 200 Test |
| 数据 |
| Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488) and SBV515 conjugated Mouse anti Human CD2 (MCA1194SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on theZE5 Cell Analyzer.. |
| 文献 |
| 1. Moingeon, P.et al.(1989) The structural biology of CD2.Immunol Rev. 111: 111-44.2. Beyers, A.D.et al.(1989) Activation of T lymphocytes via monoclonal antibodies against rat cell surface antigens with particular reference to CD2 antigen.Immunol Rev. 111: 59-77.3. Lin, C.W.et al.(2005) CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features.Blood. 106 (10): 3567-74.4. Kap, Y.S.et al.(2009) A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.J Histochem Cytochem. 57: 1159-67. |
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