Mouse anti Human CD172a antibody, clone 15-414
货号：MCA2518PE
规格：100 Tests
价格：3893
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD172a
物种：人
宿主：小鼠
抗体亚型：IgG2a
克隆号：15-414
荧光染料：PE
| 产品介绍 | | |
| Mouse anti Human CD172a antibody, clone 15-414recognizes human CD172a, also known as signal-regulatory protein alpha, a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and haematopoietic stem cells.CD172a acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. Ligand binding of CD172a to integrin-associated protein CD47, results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of CD172a, mediating the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. These then act as regulators of cellular function, through dephosphorylation of specific substrates. Ligation of CD172a with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs. |
| 产品详情 | | |
| Target Species | Mouse |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid |
| Reconstitution | Reconstitute with 1.0 ml distilled water |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 1% Bovine Serum Albumin |
| Fusion Partners | Spleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at +4oC.After reconstitution store at +4oC.DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 推荐产品 |
| Description | Product Code | Applications | Pack Size |
| Rat IgG2a Negative Control:RPE | MCA1212PE | F | 100 Tests |
| 文献 |
| 1.Fujioka, Y.et al.(1996) A novel membrane glycoprotein, SHPS-1, that binds the SH2-domain-containing protein tyrosine phosphatase SHP-2 in response to mitogens and cell adhesion.Mol Cell Biol. 16 (12): 6887-99.2.Baba, T.et al.(2012) Novel Process of Intrathymic Tumor-Immune Tolerance through CCR2-Mediated Recruitment of Sirpα(+) Dendritic Cells: A Murine Model.PLoS One. 7: e41154.3.Szaraz, P.et al.(2016)In VitroDifferentiation of First Trimester Human Umbilical Cord Perivascular Cells into Contracting Cardiomyocyte-Like CellsStem Cells International. 2016: 1-13.4.Hussen, J.et al.(2014) The chemokine CCL5 induces selective migration of bovine classical monocytes and drives their differentiation into LPS-hyporesponsive macrophagesin vitro.Dev Comp Immunol. 47 (2): 169-77. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
推荐用于 Flow
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