| 产品介绍 | | |
| Mouse anti human CD138 antibody, clone B-A38recognizes human CD138, also known as Syndecan-1 (SDC-1). CD138 is a member of the transmembrane heparan sulfate proteoglycan family (O’Connellet al.2004,Sandersonet al.2008). It is composed of a core protein (comprising 3 domains; a short cytoplasmic domain, a transmembrane domain, and a long extracellular domain) and covalently attached heparan sulfate chains (Sandersonet al.2008).Syndecan-1 is expressed on the surface of plasma cells within the hematopoietic system and on the surface of mature epithelial cells (O’Connellet al.2004). It acts as an extracellular matrix receptor, involved in many cellular functions, including cell binding, cell signaling and cytoskeletal organization through cell-cell adhesion and cell-matrix adhesion (Sandersonet al.2008). |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Reconstitution | Pack Size: 100 TestsReconstitute with 1 ml distilled waterPack Size: 25 TestsReconstitute with 0.25 ml distilled water |
| Preparation | Purified IgG prepared by ion exchange chromatography |
| Fusion Partners | Spleen cells from immunized Balb/c (Iffa Credo) mice were fused with cells of the mouse X63/Ag.8653 myeloma cell line. |
| Reconstitution | Reconstitute with 1 ml distilled water |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | U266 cell line. |
| Approx. Protein Concentrations | IgG concentration 0.1 mg/ml |
| Preservative Stabilisers | 0.09%Sodium Azide1%Bovine Serum Albumin5%Sucrose |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Pack Size: 100 TestsPrior to reconstitution store at +4℃ following reconstitution store at +4℃.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 25 TestsPrior to reconstitution store at +4℃.After reconstitution store at +4℃.DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
| 文献 |
| 1.Elbezanti, O.W.et al.(2022) Development of a Novel Bruton’s Tyrosine Kinase Inhibitor that exerts Anti-Cancer Activities Potentiates Response of Chemotherapeutic Agents In Multiple Myeloma Stem Cell-Like CellsbioRxiv 2022.03.10.483708 [Epub ahead of print].2.Borset, M.et al.(1993) Lack of IL-1 secretion from human myeloma cells highly purified by immunomagnetic separation.Br J Haematol. 85 (3): 446-51.3.Du, S.et al.(2010) Systemic mastocytosis in association with chronic lymphocytic leukemia and plasma cell myeloma.Int J Clin Exp Pathol. 3 (4): 448-57.4.Kylänpää, L.et al.(2009) Syndecan-1 and tenascin expression in cystic tumors of the pancreas.JOP. 10 (4): 378-82. |
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