产品介绍 | | |
| Rabbit anti Mouse Interleukin-2 antibodyrecognizes mouse Interleukin-2 (IL-2), a potent lymphoid cell growth factor which primarily acts on T lymphocytes, in response to antigenic or mitogenic stimulation.IL-2 stimulates the growth/differentiation of monocytes, macrophages, B lymphocytes, NK cells, and lymphokine activated killer cells which are used in experimental cancer therapy. |
产品详情 | | |
Target Species | Mouse |
Product Form | Purified IgG conjugated to Biotin - lyophilized |
| Reconstitution | Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albuminCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. |
Antiserum Preparation | Antisera to mouse IL-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
| Carrier Free | Yes |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml after reconstitution |
Immunogen | Recombinant mouse IL-2(PMP38) |
Regulatory | For research purposes only |
Guarantee | 6 months from date of despatch |
存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 0.25 | 1.0ug/ml |
Western Blotting | √ | 0.1 | 0.2ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.ELISAAAM35 may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA withAAM35Bas the detection reagent andPMP38as the standard. |
二抗相关产品 |
Description | Product Code | Applications | Pack Size |
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E;WB | 1 mg |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | Flow | 1 mg |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E;WB | 1 mg |
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C;E;WB | 1 mg |
Sheep anti Rabbit IgG:FITC | STAR34B | C;F | 1 mg |
文献 |
| 1. Smith, K.A. (1998) Interleukin-2: inception, impact, and implications.Science. 240: 1169-1176. |
QQ:1749072012