| 产品介绍 | | |
| Mouse anti SLP-76 antibody, clone SLP-76/03recognises the SH2 domain-containing leucocyte protein, also known as SLP-76 or Lymphocyte cytosolic protein 2. SLP-76 is a ~76 kDa tyrosine phosphoprotein, expressed primarily by T lymphocytes and cells of the myeloid lineage.SLP-76 is one of several adaptor proteins assisting with TCR-mediated signal transduction, through GRB2 binding, functioning as a substrate for the protein-tyrosine kinase ZAP-70 and thus playing a crucial role in both T cell ontogeny and activation. Other molecules that associate with SLP-76 include phospholipase C (PLC)–gamma, Vav, Nck and fyn binding protein. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | A fusion protein corresponding to amino acids 216-434 of human SLP-76. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Immunoprecipitation | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Di Bartolo Vet al.(2007) A novel pathway down-modulating T cell activation involves HPK-1-dependent recruitment of 14-3-3 proteins on SLP-76.J Exp Med. 204 (3): 681-91.2.Brockmeyer, C.et al.(2011) T cell receptor (TCR)-induced tyrosine phosphorylation dynamics identifies themis as a new TCR signalosome component.J Biol Chem. 286: 7535-47.3.Lasserre, R.et al.(2011) Release of serine/threonine-phosphorylated adaptors from signaling microclusters down-regulates T cell activation.J Cell Biol. 195 (5): 839-53.4.Lasserre, R.et al.(2010) Ezrin tunes T-cell activation by controlling Dlg1 and microtubule positioning at the immunological synapse.EMBO J. 29: 2301-14.5.Paster, W.et al.(2013) GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development.J Immunol. 190 (7): 3749-56. |
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