| 产品介绍 | | |
| Mouse anti Bovine interleukin-4, clone CC313is specific for bovine interleukin 4 (IL-4), also known as B-cell stimulatory factor 1. Bovine interleukin-4 is a 15 kDa, 135 amino acid peptide which is processed to a mature secreted form with the cleavage of a 24 amino acid, N-terminal signal peptide resulting in a 12.6 kDa active cytokine, the apparent MW may be altered by glycosylation (Heussleret al.1992).IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses.Mouse anti Bovine interleukin-4, clone CC313 has been used successfully as a coating antibody in a sandwich ELISA with biotinylated Mouse anti Bovine IL-4 antibody, clone CC314 (MCA2372B) as a detection reagent both with bovine (Stabelet al.2011) and ovine samples (Olivieret al.2012). Clones CC313 and CC314 have been used in the reverse configuration for the measurement of interleukin-4 in caprine samples (Marinaroet al.2012). |
| 产品详情 | | |
| Target Species | Bovine |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Fusion Partners | Spleen cells form immunized BALB/c mice were fused with cells of the SP2/0 myeloma cell line. |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Recombinant bovine IL-4. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | 2ug/ml | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Hope, J.C.et al.(2005) Development of detection methods for ruminant interleukin (IL)-4.J Immunol Methods. 301 (1-2): 114-23.2.Abbott, J.R.et al.(2005) Rapid and long-term disappearance of CD4+ T lymphocyte responses specific forAnaplasma marginalemajor surface protein-2 (MSP2) in MSP2 vaccinates following challenge with liveA. marginale.J Immunol. 174 (11): 6702-15.3.Sipka, A.et al.(2013) Prednisolone and cefapirin act synergistically in resolving experimentalEscherichia colimastitisJ Dairy Sci. 96: 4406-18.4.Stabel, J.R.et al.(2013) Disparate Host Immunity toMycobacterium aviumsubsp. paratuberculosis Antigens in Calves Inoculated withM. aviumsubsp. paratuberculosis,M. aviumsubsp. avium,M. kansasiiandM. bovis.Clin Vaccine Immunol. 20: 848-57. |
QQ:1749072012