Mouse anri Human Aurora -A kinase monoclonal antibody, clone 35C1
货号：MCA2249
规格：0.1 mg
价格：2576
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：Aurora -A kinase
物种：人
宿主：小鼠
抗体亚型：IgG2b
克隆号：35C1
荧光染料：Purified
| 产品介绍 | | |
| Mouse anri Human Aurora -A kinase monoclonal antibody, clone 35C1recognizes human Aurora-A kinase also known as Aurora 2, breast tumor-amplified kinase and serine/threonine-protein kinase 6 or 15. Aurora kinase A is member of the Ser/Thr protein kinase family containing a singleprotein kinase domain, has a molecular weight of ~46kDa and is involved in mitotic spindle assembly (Ducatet al.2004).Aurora-A kinase is reported to be overexpressed in many epithelial cancers and is thought to play an important role in tumorigenesis (Katayamaet al.2003). Aurora A kinase appears to facilitate phosphorylation of centrin and co-localizes with it at centrosomes with maximum expression through prophase to late metaphase (Lukasiewiczet al.2011).Mouse anti human Aurora-A kinase, clone 35C1 recognizes an epitope within the non-catalytic N-terminal domain of Aurora-A. Clone 35C1 does not inhibit Aurora-A kinase activity (Cremetet al.2003). |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Concentrated Tissue Culture Supernatant - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Fusion Partners | Spleen cells from immunized Balb/c were fused with cells of the SP2/0-Ag14 mouse myeloma cell line |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Recombinant Aurora-A |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Preservative Stabilisers | 0.01%Sodium Azide |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Cremet, J.Y.et al.(2003) Preparation and characterization of a human aurora-A kinase monoclonal antibody.Mol Cell Biochem. 243 (1-2): 123-31.2.Hartsink-Segers SAet al.(2013) Inhibiting Polo-like kinase 1 causes growth reduction and apoptosis in pediatric acute lymphoblastic leukemia cells.Haematologica. 98 (10): 1539-46.3.Hartsink-Segers, S.A.et al.(2013) Aurora kinases in childhood acute leukemia: the promise of aurora B as therapeutic target.Leukemia. 27: 560-8.4.Lukasiewicz, K.B.et al.(2011) Control of centrin stability by Aurora A.PLoS One. 6: e21291. |
技术参数 产品应用 WB;IF
产品类型 Monoclonal Antibody
推荐用于 WB;IF
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