产品介绍 | | |
| Mouse anti Human CD146 antibody, clone OJ79crecognizes human Cell surface glycoprotein MUC18, also known as CD146, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule (MCAM) or S-endo 1 endothelial-associated antigen. CD146 is a 646 amino acid single pass type 1 transmembrane glycoprotein with a calculated molecular mass of ~72 kDa. However due to extensive N-linked glycosylation CD146 migrates in polyacrylamide gels with an apparent molecular mass of ~118 kDa. CD146 is a member of the immunoglobulin superfamily bearing 2V-type Ig-likeand 3C-type Ig-like domains. CD146 is expressed by all endothelial cells and by melanoma cells and appears to act as an adhesion molecule (UniProt: P43121). Expression in melanoma may be linked to tumor progression (Lehmannet al.1989).Mouse anti Human CD146 antibody, clone OJ79c is highly expressed on pericytes and has been utilized for the identification of perivascular mesenchymal precursor cells from cardiac muscle using flow cytometry (Chenet al.2014). |
| 产品详情 | | |
Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Pig | √ |
| Dog | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | Pack Size: 25 µg 0.09% Sodium Azide Pack Size: 0.2 mg 0.09% Sodium Azide (NaN3) |
| Carrier Free | Yes |
| Immunogen | Recombinant human MUC18 (D1-D5) Fc protein. |
| Approx. Protein Concentrations | IgG concentration1.0 mg/ml |
| Fusion Partners | Spleen cells from immunised mice were fused with cells of the mouse Sp2/0 Ag.14 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/10 | 1/50 |
| ELISA | √ | | |
| Immunofluorescence | √ | | |
| Immunohistology - Frozen | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
- Histology Positive Control TissueMelanoma
|
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| 文献 |
| 1. James, A.W.et al.(2017) Isolation and characterization of canine perivascular stem/stromal cells for bone tissue engineering.PLoS One. 12 (5): e0177308.2. Shen, J.et al.(2018) Effects of WNT3A and WNT16 on the Osteogenic and Adipogenic Differentiation of Perivascular Stem/Stromal Cells.Tissue Eng Part A. 24 (1-2): 68-80.3. Lapin, M.et al.(2017) Single-cell mRNA profiling reveals transcriptional heterogeneity among pancreatic circulating tumour cells.BMC Cancer. 17 (1): 390.4. Esteves, C.L.et al.(2017) Isolation and characterization of equine native MSC populations.Stem Cell Res Ther. 8 (1): 80.5. Gaceb, A.et al.(2017) Pericytes secrete pro-regenerative molecules in response to platelet-derived growth factor-BB.J Cereb Blood Flow Metab. : 271678X17719645.6. Noda, S.et al.(2019) Effect of cell culture density on dental pulp-derived mesenchymal stem cells with reference to osteogenic differentiation.Sci Rep. 9 (1): 5430.7. Gaceb, A.et al.(2020) AnIn Vitro.Partial Lesion Model of Differentiated Human Mesencephalic Neurons: Effect of Pericyte Secretome on Phenotypic Markers.J Mol Neurosci. 70 (11): 1914-25.8. Stefanska, A.et al.(2021) Role of Pericytes in the Development of the Renin/Angiotensin System: Induction of Functional Renin in Cultures of Pericytes.Methods Mol Biol. 2235: 169-180.9. Orikasa, S.et al.(2022) Hypoxia-inducible factor 1α induces osteo/odontoblast differentiation of human dental pulp stem cells via Wnt/β-catenin transcriptional cofactor BCL9.Sci Rep. 12 (1): 682. |
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