Mouse anti Huntingtin antibody, clone HDC8A4
货号：MCA2051
规格：0.1 mg
价格：2576
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：Huntingtin
物种：人/小鼠
宿主：小鼠
抗体亚型：IgG1
克隆号：HDC8A4
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti Huntingtin antibody, clone HDC8A4reacts with an epitope corresponding to the HDC region (2703 - 2911 amino acids) of the huntingtin protein.Mouse anti Huntingtin antibody, clone HDC8A4 detects a ~350 kDa band on western blots but also detects smaller degradation products of huntingtin. Clone HDC8A4 recognizes both denatured and native huntingtin in human brain.The combined use of Mouse anti Huntingtin antibody, clone HDC8A4 (MCA2051) and HDB4E10 (MCA2050) demonstrate that huntingtin is enriched in neuronal cells in the brain. |
| 产品详情 | | |
| Species Cross-Reactivity | Human |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G. |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Recombinant protein corresponding to amino acids 2703 - 2911 of huntingtin. |
| Preservative Stabilisers | 0.09%Sodium Azide |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Western Blotting | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Wilkinson, F. L.et al. (1999) Localization of rabbit huntingtin using a new panel of monoclonal antibodies.Molecular Brain Research. 69: 10-20.2.Yao Yet al.(2015) A striatal-enriched intronic GPCR modulates huntingtin levels and toxicity.Elife. 4: 4.3.Jones, A.L. (1999) The localization and interactions of huntingtin.Philos Trans R Soc Lond B Biol Sci. 354 (1386): 1021-7. |
技术参数 产品应用 IP;WB
产品类型 Monoclonal Antibody
推荐用于 IP;WB
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