Mouse anti Tubulin beta 3 antibody, clone TU-20
货号：MCA2047F
规格：0.1 mg
价格：6035
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：Tubulin beta 3
物种：人
宿主：小鼠
抗体亚型：IgG1
克隆号：TU-20
荧光染料：FITC
| 产品介绍 | | |
| Mouse anti Tubulin beta 3 antibody, clone TU-20recognizes class III beta-tubulin, restricted to neuronal tissue (Leandro-García 2010;Katsetos 2003).Mouse anti Tubulin beta 3 antibody, clone TU-20 has been used to investigate tumors of neuronal origin (Jirásek 2002) including neuroblastoma (Prasannan 2000)and ganglioneuroma (Dráberová 1998). Class III beta tubulin is highly expressed in tumors of neuronal origin rather than in non-neuronal tumors (Person 2017). |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
| Preparation | Purified IgG prepared by DEAE chromatography |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09%Sodium Azide |
| Immunogen | Synthetic peptide, ESESQGPK, corresponding to amino acids 441-448 of human class III beta tubulin coupled to Keyhole Limpet Hemocyanin (KLH). |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| FITC | 490 | 525 |
| Approx. Protein Concentrations | IgG concentration 0.1mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Immunofluorescence | √ | 1/10 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Dráberová, E.et al.(1998) Expression of class III beta-tubulin in normal and neoplastic human tissues.Histochem Cell Biol. 109 (3): 231-9.2.Hattermann, K.et al.(2010) The chemokine receptor CXCR7 is highly expressed in human glioma cells and mediates antiapoptotic effects.Cancer Res. 70: 3299-308.3.Rosito, M.et al.(2012) CXCL16 Orchestrates Adenosine A3 Receptor and MCP-1/CCL2 Activity to Protect Neurons from Excitotoxic Cell Death in the CNS.J Neurosci. 32: 3154-63.4.Nicot, A. and DiCicco-Bloom, E. (2001) Regulation of neuroblast mitosis is determined by PACAP receptor isoform expression.Proc Natl Acad Sci U S A. 98: 4758-63.5.Pěknicová, J.et al.(2001) Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail.Biol Reprod. 65: 672-9. |
技术参数 产品应用 IF
产品类型 Monoclonal Antibody
推荐用于 IF
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