mouse anti Rat IgM HEAVY chain
货号：MCA189
规格：0.25 mg
价格：3341
产品类型：流式抗体
品牌：Bio-Rad
抗原： IgM
物种：大鼠
宿主：小鼠
抗体亚型：IgG1, kappa
克隆号：MARM-4
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti Rat IgM Heavy Chain antibody, clone MARM-4recognizes the mu heavy chain of rat immunoglobulin and does not cross-react with other immunoglobulin classes. |
| 产品详情 | | |
Target Species | Rat |
| Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.1% Sodium Azide |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
| Immunogen | IR202 rat IgM myeloma protein |
| Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | 5ug/ml as coating antibody |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- ELISAThis antibody may be used as a coating antibody in a sandwich ELISA in combination with clone MARK-1/MARL-15 (Product code MCA1296P) as detection reagent and purified rat IgM (Product code PRP08).
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| 文献 |
| 1. Bazin, H.et al.(1974) Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c.Eur J Immunol. 4 (1): 44-8.2. Bazin, H.et al.(1978) Transplantable IgD immunoglobulin-secreting tumors in rat.J Immunol. 121 (5): 2077-82.3. Khalifeh, M.S.et al.(2010) Investigation ofthe role of tumour necrosis factor-{alpha}, interleukin-1{beta}, interleukin-10, nitric oxide and rheumatoid factor-immunoglobulin M in a rat model of arthritis.Lab Anim. 44: 143-9.4. Bézie, S.et al.(2015) Compensatory Regulatory Networks between CD8 T, B, and Myeloid Cells in Organ Transplantation Tolerance.J Immunol. 195 (12): 5805-15.5. Ueta, H.et al.(2018) Single blood transfusion induces the production of donor-specific alloantibodies and regulatory T cells mainly in the spleen.Int Immunol. 30 (2): 53-67.6. Tulinská, J.et al.(2018) Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90days (EU 7th Framework Programme project GRACE).Arch Toxicol. 92 (7): 2385-99. |
技术参数 产品应用 ELISA
产品类型 Monoclonal Antibody
推荐用于 ELISA
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