Mouse anti Sheep GM-CSF, clone 3C2
货号：MCA1924
规格：0.5 mg
价格：3970
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：GM-CSF
物种：绵羊
宿主：小鼠
抗体亚型：IgG1
克隆号：3C2
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti Sheep GM-CSF, clone 3C2recognizes both recombinant and native ovine Granulocyte-macrophage colony-stimulating factor, also known GM-CSF or CSF, a 16.3kDa cytokine responsible for the stimulation and growth of cells from granulocyte, macrophage, eosinophil and erythrocyte hematopoietic precursor cell linages.Mouse anti Sheep GM-CSF, clone 3C2 has is of particular interest as it has been shown to reconize GM-CSF in cattle while other clones such as 8D8 do not crosss react with cattle..Mouse anti Sheep GM-CSF, clone 3C2 has been reported to neutralise the activity of both recombinant ovine and native GM-CSF.Removal of sodium azide is recommended prior to use in functional assays. |
| 产品详情 | | |
| Target Species | Sheep |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09%Sodium Azide |
| Immunogen | CHO expressed recombinant ovine GM-CSF. |
| Fusion Partners | Spleen cells from immunised Balb/c mice were fused with cells of the NSO myeloma cell line. |
| Acknowledgements | This reagent was evaluated at the Moredun Research Institute and The Roslin Institute at the University of Edinburgh as part of ‘The route to identification of immunological correlates of protection in ruminants’ Industrial Partnership Award funded by BBSRC (grant numbers BB/I019863/1; BB/I020519/1) with the support of Bio-Rad. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Scott, J.L.et al.(2007) Granulocyte-macrophage colony stimulating factor and interleukin-8 in the reproductive tract of ewes following oestrus and mating.Reprod Fertil Dev. 19: 585-93.2.Entrican, G.et al.(1996) Development of a sandwich ELISA for ovine granulocyte/macrophage colony-stimulating factor.Vet Immunol Immunopathol. 50 (1-2): 105-15.3.Deane, D.et al.(2000) Orf virus encodes a novel secreted protein inhibitor of granulocyte-macrophage colony-stimulating factor and interleukin-2.J Virol. 74: 1313-20.4.Foulon, E. and Foucras, G. (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.J Immunol Methods. 339: 1-10. |
技术参数 产品应用 Elisa;WB
产品类型 Monoclonal Antibody
推荐用于 Elisa;WB
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