CD40 Monoclonal Antibody (HM40-3), Functional Grade, eBioscience™/CD40单抗 (HM40-3), 功能抗体, eBioscience™

货号：16-0402-82,16-0402-81,16-0402-85,16-0402-86,16-0402-38,16-0402-025,16-0402-050

规格：100 µg,50 µg,500 µg,1 mg,5 mg,25 mg,50 mg

价格：1992,1156,3274,6076,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD40

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgM

克隆号： HM40-3

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	IgM, kappa
浓度：	1 mg/mL	用法：	1 µg/test（Flow）

产品详细信息Description: The HM40-3 monoclonal antibody reacts with mouse and rat CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.Applications Reported: The HM40-3 antibody has been reported for use in flow cytometric analysis. HM40-3 has also been reported in inhibition of binding of soluble CD154 to CD40 and in vitro activation of B cells.Applications Tested: The HM40-3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息The CD40 antigen is a single chain glycoprotein that is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin's disease associated antigen, CD30. CD40 is present on all B cells except plasma cells and is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of CD40, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction. The interaction of CD40 and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported. Diseases associated with CD40 dysfunction include Type 3 Hyper-Igm immunodeficiency and CD40 ligand deficiency.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD40 Antibody (16-0402-82) in FlowStaining of BALB/c splenocytes with 0.5 µg of FG Armenian Hamster IgG isotype control (Product # 16-4888-81) (open histogram) or 0.5 µg of Anti-Mouse/Rat CD40 Functional Grade Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC (Product # 11-4111).Total viable cells were used for analysis.

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参考文献：

流式1.Cell reportsDistinct Requirements of CHD4 during B Cell Development and Antibody Response."16-0402 was used in Flow cytometry/Cell sorting to reveal distinct roles for CHD4 in B cell development and CSR and links the H3K9me3 epigenetic mark with AID recruitment to the Igh locus."AuthorsYen WF,Sharma R,Cols M,Lau CM,Chaudhry A,Chowdhury P,Yewdell WT,Vaidyanathan B,Sun A,Coffre M,Pucella JN,Chen CC,Jasin M,Sun JC,Rudensky AY,Koralov SB,Chaudhuri J2.Molecular and cellular biologyApurinic/apyrimidinic endonuclease 1 is the essential nuclease during immunoglobulin class switch recombination.AuthorsMasani S,Han L,Yu K功能检测3.Frontiers in immunologySemaphorin 4C: A Novel Component of B-Cell Polarization in Th2-Driven Immune Responses."16-0402 was used in Functional assays to report that Semaphorin 4C is inducible in human and murine B-cells and may be important for normal B-cell development."AuthorsXue D,Desjardins M,Kaufman GN,Béland M,Al-Tamemi S,Ahmed E,Tao S,Friedel RH,Mourad W,Mazer BD4.Cell researchThe RNA-binding protein ROD1/PTBP3 cotranscriptionally defines AID-loading sites to mediate antibody class switch in mammalian genomes."16-0402 was used in Functional assays to identify an RNA-binding protein that is both required and sufficient to define activation-induced cytidine deaminase-binding sites genome-wide in activated B cells."AuthorsChen J,Cai Z,Bai M,Yu X,Zhang C,Cao C,Hu X,Wang L,Su R,Wang D,Wang L,Yao Y,Ye R,Hou B,Yu Y,Yu S,Li J,Xue Y5.Frontiers in immunologyA Complex Relationship between TRAF3 and Non-Canonical NF-κB2 Activation in B Lymphocytes."16-0402 was used in Immunoprecipitation to conclude that there is not a simple linear relationship in B lymphocytes between relative levels of cellular TRAF3, induced TRAF3 degradation, NIK activation, and NF-κB2 activation."AuthorsLin WW,Hildebrand JM,Bishop GA

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