| 产品介绍 | | |
| Mouse anti Human CD326 antibody, clone VU-1D9recognizes epithelial cell adhesion molecule (Ep-CAM), a ~34 kDa cell surface antigen otherwise known as CD326, MOC31 or Ber-EP4. CD326 is a type 1 transmembrane glycoprotein, expressed on the basolateral cell membrane of the majority of epithelial tissues, with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells.Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells, andde novoexpression being seen in dysplastic squamous epithelium. |
| 产品详情 | | |
Target Species | Human |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Reconstitution | Reconstitute with 1.0 ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution |
Preparation | Purified IgG prepared by affinity chromatography on Protein A |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin 5% Sucrose |
| Immunogen | HG9 cell line (small cell lung carcinoma) |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
| Fusion Partners | Spleen cells from immunised mice were fused with cells of the mouse SP2/0 myeloma cell line |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
| RPE 561nm laser | 546 | 578 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at +4oC.After reconstitution store at +4oC.DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | 1/10 |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 1x106cells in 100u
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| 数据 |
| Figure A. FITC conjugated Mouse anti Human CD49f (MCA699F) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated Mouse anti Human CD49f (MCA699F) and Alexa Fluor 647 conjugated Mouse anti Human CD326 (MCA1870A647). All experiments performed on HT-29 cells gated on live single cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer |
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:RPE | MCA928PE | Flow | 100 Tests |
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| 文献 |
| References for CD326 antibody1. Koga, Y.et al.(2008) Improved recovery of exfoliated colonocytes from feces using newly developed immunomagnetic beads.Gastroenterol Res Pract. 2008: 605273.2. Fillmore, C.M.et al.(2010) Estrogen expands breast cancer stem-like cells through paracrine FGF/Tbx3 signaling.Proc Natl Acad Sci U S A. 107 (50): 21737-42.3. Murakata, A.et al.(2011) Gene expression signature of the gross morphology in hepatocellular carcinoma.Ann Surg. 253: 94-100.4. Keller, P.J.et al.(2012) Defining the cellular precursors to human breast cancer.Proc Natl Acad Sci U S A. 109 (8): 2772-7.5. Jeong, H.T.et al.(2012) MRI features of hepatocellular carcinoma expressing progenitor cell markers.Liver Int. 32: 430-40.6. Armstrong, A.J.et al.(2019) Pharmacodynamic study of radium-223 in men with bone metastatic castration resistant prostate cancer.PLoS One. 14 (5): e0216934.Further Reading7. Winter, M.J.et al.(2003) The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology.Am J Pathol. 163 (6): 2139-48. |
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