| 产品介绍 | | |
| Hamster anti Mouse CD79b antibody, clone HM79-11recognizes murine CD79 beta, expressed by B cells as part of the B cell receptor complex (immunoglobulin and the CD79 alpha/beta heterodimer).Murine CD79 beta is expressed at the cell surface of pro-B cells prior to surface immunoglobulin, and is expressed throughout B cell differentiation.CD79 beta is a B cell specific marker, valuable for the detection of B cells at all maturation stages. |
| 产品详情 | | |
Target Species | Mouse |
| Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide |
| Carrier Free | Yes |
| Immunogen | CD79 alpha/CD79 beta heterodimer purified from WEHI-231 B cells. |
| Fusion Partners | Lymph node cells from immunised Armenian hamsters were fused with cells of the mouse X63-Ag8.653 myeloma cell line. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | 1/50 | 1/200 |
| Immunohistology - Frozen | √ | | |
| Immunoprecipitation | √ | | |
| Western Blotting | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/postive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul whole blood
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| 同型 |
| Description | Product Code | Applications | Pack Size |
Hamster (Armenian) IgG Negative Control | MCA2356 | F | 100 Tests |
| 文献 |
| 1. Koyama, M.et al.(1997) CD79 alpha/CD79 beta heterodimers are expressed on pro-B cell surfaces without associated mu heavy chain.Int Immunol. 9 (11): 1767-72.2. Luger, D.et al.(2013) Expression of the B-cell receptor component CD79a on immature myeloid cells contributes to their tumor promoting effects.PLoS One. 8 (10): e76115.3. Jelínek, F.et al.(2005) Characteristics of two mouse bcr-abl-transformed cell lines. II. Pathological lesions induced in mice.Folia Biol (Praha). 51 (4): 93-102.4. Ankeny, D.P.et al.(2006) Spinal cord injury triggers systemic autoimmunity: evidence for chronic B lymphocyte activation and lupus-like autoantibody synthesis.J Neurochem. 99 (4): 1073-87.5. Lacoste-collin, L.et al.(2007) Effect of continuous irradiation with a very low dose of gamma rays on life span and the immune system in SJL mice prone to B-cell lymphoma.Radiat Res. 168 (6): 725-32. |
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