Rat anti Human dopamine transporter antibody, clone DAT-Nt
货号：0100-0075
规格：0.1 ml
价格：8058
产品类型：免疫组化一抗
品牌：Bio-Rad
物种：人
宿主：大鼠
抗体亚型：IgG2a
克隆号：DAT-Nt(4F8)
荧光染料：Purified
| 产品介绍 | | |
| Rabbit anti Human dopamine transporter antibodyrecognizes an epitope within the C-terminal region of dopamine transporter (DAT), also known as solute carrier family 6.DAT is principally expressed in the presynaptic membrane of dopaminergic nerve terminals, where it mediates the rapid removal of dopamine from the synaptic cleft after neuronal stimulation. As such, DAT plays an important role in regulating the availability of dopamine in the synaptic cleft and thus in modulating the physiological functions of dopamine.The reuptake of dopamine by DAT is inhibited by psychostimulants such as cocaine and amphetamine. |
| 产品详情 | | |
| Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Monkey | √ |
| Mouse | √ |
| Product Form | Purified IgG - liquid |
| Buffer Solution | 10mM HEPES pH7.5150mM NaCl |
| Immunogen | Purified mouse IgG, IgA and IgM. |
| Approx. Protein Concentrations | IKeyhole limpet haemocyanin conjugated synthetic peptide corresponding to the C-terminus region (CT) of human dopamine transporter. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Immunohistology - Frozen | √ | | 1/1000 |
| Western Blotting | √ | | 1/1000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Western BlottingAHP932 detects a band of approximately 88kDa in human caudate cell lysates. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E,WB | 1mg |
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | E,WB | 1mg |
| 文献 |
| 1.Ding, G.et al.(2010) Effect of cryopreservation on biological and immunological properties of stem cells from apical papilla.J Cell Physiol. 22: 415-22.2.Shao, L.et al.(2015) ComparativeIn VitroandIn VivoStudies of Porcine Rotavirus G9P[13] and Human Rotavirus Wa G1P[8].J Virol. 90 (1): 142-51.3.Iver, S.et al.(2019) Probing BAK and BAX Activation and Pore Assembly with Cytochrome c Release, Limited Proteolysis, and Oxidant-Induced Linkage.Methods Mol Biol. 1877: 201-16. |
技术参数 产品应用 WB
产品类型 Monoclonal Antibody
推荐用于 WB
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