| 产品介绍 | | |
| Mouse anti Human CD66e antibody, clone C365D3 (NCRC23)recognizes human Carcinoembryonic antigen-related cell adhesion molecule 5, also known as CD66e, carcinoembryonic antigen, Meconium antigen 100, CEA or CEACAM5. CD66e is a 702 amino acid ~77 kDa GPI anchored membrane protein containing 7Ig-like domains. Mouse anti Human CD66e antibody, clone C365D3 does not cross-react with normal cross-reacting antigen (CD66c), or with biliary glycoprotein 1 (CD66a) as indicated by binding assays (Price 1988, note: in this study Mouse anti Human CD66e antibody, clone C365D3 is designated as clone 6 (from author)). |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Preparation | - MCA1744: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- MCA1744T: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
|
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09%Sodium Azide |
| Carrier Free | Yes |
| Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3NSI myeloma cell line. |
| Approx. Protein Concentrations | IgG concentration 1 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| Immunohistology - Paraffin | √ | | 1/1000 |
| Immunohistology - Frozen | √ | | 1/1000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5mg |
| Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) | STAR117A | E,WB | 0.5mg |
| 文献 |
| 1.Seth, J.et al.(1988) Carcinoembryonic antigen.Lancet. 1 (8599): 1399.2.Soucek, K.et al.(2010) Fetal colon cell line FHC exhibits tumorigenic phenotype, complex karyotype, and TP53 gene mutation.Cancer Genet Cytogenet. 197: 107-16.3.Kalinina, T.et al.(2010) Establishment and characterization of a new human pancreatic adenocarcinoma cell line with high metastatic potential to the lung.BMC Cancer.10: 295.4.Dallas, M.R.et al.(2012) Divergent roles of CD44 and carcinoembryonic antigen in colon cancer metastasis.FASEB J. 226: 2648-56. |
QQ:1749072012