Mouse anti Human CD31:FITC
货号：MCA1738FT,MCA1738F
规格：25 µg,0.1 mg
价格：893,2865
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD31
物种：人
宿主：小鼠
抗体亚型：IgG1
克隆号：WM59
荧光染料：FITC
| 产品介绍 | | |
| Mouse anti Human CD31 monoclonal antibody, clone WM59recognizes the human CD31 antigen, a ~130 kDa single pass type I transmembrane glycoprotein bearing sixC2 immunoglobulin domains. CD31 is expressed by all continuous endothelia including arteries, veins and non-sinusoidal capillaries, platelets,granulocytes and some lymphocytes. CD31 is not expressed by discontinuous endothelia such as hepatic sinusoids and splenic red pulp (Mulleret al.1989).CD31 is also known as PECAM-1.The binding epitope for mouse anti human CD31, clone WM59 has been mapped to the Ig-like domain 2 (Fawcettet al.1995). |
| 产品详情 | | |
| Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Cynomolgus monkey | √ |
| Rhesus Monkey | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Carrier Free | Yes |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1%Bovine Serum Albumin |
| Approx. Protein Concentrations | IgG concentration 0.1 mg/ml |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| FITC | 490 | 525 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | 1/2Pack Size: 0.1 mgNeatPack Size: 25 µg |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 同型对照 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
| 文献 |
| 1.Cabañas, C.et al.(1989) Characterization of a novel myeloid antigen regulated during differentiation of monocytic cells.Eur J Immunol. 19 (8): 1373-8.2.Fawcett, J.et al. (1995) Mapping the homotypic binding sites in CD31 and the role of CD31 adhesion in the formation of interendothelial cell contacts.J Cell Biol. 128:1229-41.3.Paul, G.et al.(2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.PLoS One. 7: e35577. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
推荐用于 Flow
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