Mouse anti Bovine CD205:FITC
货号：MCA1651F
规格：0.1 mg
价格：3851
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD205
物种：Bovine
宿主：小鼠
抗体亚型：IgG2b
克隆号：CC98
荧光染料：FITC
| 产品介绍 | | |
| Mouse anti Bovine CD205 antibody, clone CC98recognizes the bovine CD205 cell surface antigen, a ~210-220 kDa molecule expressed by T cells that are CD2+ve but not WC1+ve. CD205 is also expressed by B cells, and weakly stains B cell follicles.Bovine CD205 has previously been described as the WC6 antigen (Gliddonet al.2004).Dendritic cells (veiled cells) in afferent lymph are strong expressors of CD205 as are dendritic cells in various other tissues. |
| 产品详情 | | |
| Target Species | Bovine |
| Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Bovine thymocytes |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Approx. Protein Concentrations | IgG concentration 0.1 mg/ml |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| FITC | 490 | 525 |
| Fusion Partners | Spleen cells from immunised BALB/c micewere fused with cells of the NS1 myeloma cell line |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | 1/10 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).Vet Immunol Immunopathol. 39 (1-3): 25-47.2.Howard, C.J.et al.(1996) Afferent lymph veiled cells stimulate proliferative responses in allogeneic CD4+ and CD8+ T cells but not gamma delta TCR+ T cells.Immunology. 88 (4): 558-64.3.Naessens, J.et al.(1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.Vet Immunol Immunopathol. 39 (1-3): 283-90. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
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