| 产品介绍 | | |
| Mouse anti Bovine CD45RB, clone CC76,recognizes the RB isoform of the bovine leucocyte common antigen expressed on subpopulations of CD4+ and CD8+ T cells. Expression levels of CD45RB are used as a marker for differentiating naïve and memory T lymphocytes (Howard C.et al., 1991). The antigen recognized by clone CC76 is also expressed on B cells but is absent from both macrophages and granulocytes. |
| 产品详情 | | |
| Target Species | Bovine |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A |
| Buffer Solution | Phosphate buffered saline |
| Carrier Free | Yes |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Immunogen | Bovine thymocytes |
| Fusion Partners | Spleen cells from immunised BALB/c micewere fused with cells of the NS1 myeloma cell line |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/50 | 1/100 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 100 Tests/1ml |
| 文献 |
| 1.Howard, C.J.et al.(1991) Distinction of naive and memory BoCD4 lymphocytes in calves with a monoclonal antibody, CC76, to a restricted determinant of the bovine leukocyte-common antigen, CD45.Eur J Immunol. 21 (9): 2219-26.2.Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).Vet Immunol Immunopathol. 39 (1-3): 25-47. |
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