Rat anti Mouse ART2.2 antibody, clone Nika102
货号:MCA1975
规格:0.25 mg
价格:3043
产品类型:流式抗体
品牌:Bio-Rad
抗原:ART2.2
物种:小鼠
宿主:大鼠
抗体亚型:IgG2a
克隆号:Nika102
荧光染料:Purified
产品介绍 | | |
Rat anti Mouse ART2.2 antibody, clone Nika102recognizes the gene product of the mouse ADP-ribosyltransferase (ART) 2.2 gene, an ortholog of rat RT6. ART2.2 expressed exclusively by mature T-cells. Levels of ART2.2 expression vary between inbred strains of mice and is absent in NZW mice, in which the ART2.2 gene is deleted (Koch-Nolteet al.1999). |
产品详情 | | |
Target Species | Mouse |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
Immunogen | pME.CD8LF-ART2.2 |
Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line. |
Approx. Protein Concentrations | IgG concentration 0.5 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 | | |
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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文献 |
1.Koch-Nolte, F.et al.(1999) A new monoclonal antibody detects a developmentally regulated mouse ecto-ADP-ribosyltransferase on T cells: subset distribution, inbred strain variation, and modulation upon T cell activation.J Immunol. 163 (11): 6014-22.2.Adriouch, S.et al. (2001) Rapid induction of naive T cell apoptosis by ecto-nicotinamide adenine dinucleotide: requirement for mono(ADP-ribosyl)transferase 2 and a downstream effector.J Immunol. 167:196-2033.Kahl, S.et al. (2000) Metalloprotease-mediated shedding of enzymatically active mouse ecto-ADP-ribosyltransferase ART2.2 upon T cell activation.J Immunol. 165:4463-9. |
技术参数 产品应用 FLow;IP
产品类型 Monoclonal Antibody
推荐用于 FLow;IP
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