产品介绍 | | |
| Mouse anti Human CD20 antibody, clone 2H7recognizes the human CD20 cell surface antigen, a 33-37 kDa non-glycosylated phosphoprotein.The CD20 antigen is expressed during pre-B-cell development. It is present on both resting and activated B-cells but is lost prior to terminal B-cell differentiation into plasma cells.The epitope recognized by clone 2H7 has been mapped to the following sequence found in the large extracellular loop of human CD20: YNCEPANPSEKNSPST. Furthermore it appears that Mouse anti Human CD20 antibody, clone 2H7 only recognizes human CD20 in its native oligomeric form (Polyaket al.2002). |
| 产品详情 | | |
Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Rhesus Monkey | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Reconstitution | Reconstitute with 1 ml distilled water |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
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存储条件 | | |
| Prior to reconstitution store at +4oC.After reconstitution store at +4oC.DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | 1/20 |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 106cells or cells or 100ul whole blood.
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| 数据 |
| Figure A. PE conjugated Mouse anti Human CD20 (MCA1710PE). Figure B. PE conjugated Mouse anti Human CD20 (MCA1710PE) and StarBright Violet 440 conjugated Mouse anti Human CD19 (MCA1940SBV440). All experiments performed on red blood lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer. |
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Description | Product Code | Applications | Pack Size |
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| 文献 |
| 1. Greig, B.et al.(2014) Stabilization media increases recovery in paucicellular cerebrospinal fluid specimens submitted for flow cytometry testing.Cytometry B Clin Cytom. 86: 135-8.2. van den Akker, E.et al.(2010) The majority of the in vitro erythroid expansion potential resides in CD34(-) cells, outweighing the contribution of CD34(+) cells and significantly increasing the erythroblast yield from peripheral blood samples.Haematologica. 95: 1594-8.3. Jaramillo, M.C.et al.(2015) Manganese (III) meso-tetrakis N-ethylpyridinium-2-yl porphyrin acts as a pro-oxidant to inhibit electron transport chain proteins, modulate bioenergetics, and enhance the response to chemotherapy in lymphoma cells.Free Radic Biol Med. 83: 89-100.4. Cecchinato, V.et al.(2017) Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization.J Immunol. 198 (1): 184-195.5. Kohler, S.L.et al.(2016) Germinal Center T Follicular Helper Cells Are Highly Permissive to HIV-1 and Alter Their Phenotype during Virus Replication.J Immunol. 196 (6): 2711-22.6. Grobárová Vet al.(2016) Quambalarine B, a Secondary Metabolite fromQuambalaria cyanescenswith Potential Anticancer Properties.J Nat Prod. 79 (9): 2304-14.7. Popov, J.et al.(2017) Unique therapeutic properties and preparation methodology of multivalent rituximab-lipid nanoparticles.Eur J Pharm Biopharm. 117: 256-69.8. Sieg, M.et al.(2019) A New Genotype of Feline Morbillivirus Infects Primary Cells of the Lung, Kidney, Brain and Peripheral Blood.Viruses. 11 (2) Feb 09 [Epub ahead of print]. |
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