Mouse anti Human RAD51 antibody
货号：VMA00534
规格：100μl
价格：2916
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：RAD51
物种：人
宿主：小鼠
抗体亚型：IgG1, kappa
克隆号：AB02/1A3
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti Human RAD51 antibodyrecognizes the DNA repair protein RAD51 homolog 1, also known as BRCA1/BRCA2-containing complex,subunit 5, RAD51 homolog A, RecA E. coli or recombination protein A.The protein encoded by RAD51 gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for RAD51 (provided by RefSeq, Aug 2009).Mouse anti Human RAD51 antibody detects a band of 37 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Preparation | Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant. |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3). |
| Immunogen | E. coli-derived recombinant human RAD51 (amino acids 1 - 339) |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml. |
| Acknowledgements | PrecisionAb is a trademark of Bio-Rad Laboratories. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch. |
| 存储条件 | | |
| Store undiluted at -20°C, avoiding repeated freeze thaw cycles. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Western Blotting | √ | 1/1000 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood. |
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技术参数 产品应用 WB
产品类型 单克隆抗体
推荐用于 wb
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