NK1.1 Monoclonal Antibody (PK136), Functional Grade, eBioscience™/NK1.1 单克隆 (PK136), 功能抗体, eBioscience™

货号：16-5941-82,16-5941-85,16-5941-38

规格：100 µg,500 µg,5 mg

价格：1781,3530,24836

产品类型：流式抗体

品牌：eBioscience

抗原：NK1.1

物种：小鼠

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：PK136

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）;Assay-Dependent（功能检测）

产品详细信息Description: The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. Several commonly used laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H and A do not express the NK1.1 antigen. For detection of NK cells in these strains the monoclonal antibody DX5 (Cat. No. 14-5971) should be used. Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells.Applications Reported: PK136 has been reported for use in flow cytometric analysis, depletion of NK cells, and in vitro functional studies.Applications Tested: The PK136 antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes and can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in other applications.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息NK1.1 (Killer cell lectin-like receptor subfamily B, member 1, KLRB1, NKR-P1A, CD161, cluster of differentiation 161), refers to Natural Killer (NK) cells, lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein, NKR-P1A or CD161, is classified as a type II membrane protein because it has an external C terminus. NKR-P1A, the receptor encoded by the KLRB1 gene, recognizes Lectin Like Transcript-1 (LLT1) as a functional ligand.仅用于科研。不用于诊断过程。

数据

NK1.1 Antibody (16-5941-82) in FlowStaining of C57BL/6 mouse splenocytes with Anti-Mouse CD49b (Integrin alpha 2) FITC (Product # 11-0491-82) and Anti-Mouse NK-1.1 PE. Total viable cells were used for analysis.

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参考文献：

流式1.AtherosclerosisExvivo culture of human atherosclerotic plaques: A model to study immune cells in atherogenesis."Published figure using CD16 monoclonal antibody (Product # 16-0168-85) in Flow Cytometry"2.Nature communicationsAntitumor immunity augments the therapeutic effects of p53 activation on acute myeloid leukemia."Published figure using NK1.1 monoclonal antibody (Product # 16-5941-82) in Flow Cytometry"3.JCI insightProgranulin prevents regulatory NK cell cytotoxicity against antiviral T cells."Published figure using NK1.1 monoclonal antibody (Product # 16-5941-82) in Flow Cytometry"4.Cancer researchMigratory and antigen presentation functions of IFN-producing killer dendritic cells."16-5941 was used in Flow cytometry/Cell sorting to study how BM-IKDCs are capable of cytotoxic killing of tumour targets, and also of potent antigen presentation after encountering antigen in the context of a viable target cell."AuthorsHimoudi N,Yan M,Bouma G,Morgenstern D,Wallace R,Seddon B,Buddle J,Eddaoudi A,Howe SJ,Cooper N,Anderson JIHC5.Journal of immunology (Baltimore, Md. : 1950)TLR3, TRIF, and caspase 8 determine double-stranded RNA-induced epithelial cell death and survival in vivo."16-5941 was used in Immunohistochemistry to conclude that dsRNA activation of the TLR3-TRIF-caspase 8 signalling pathway in intestinal epithelial cells is host protective during viral infection."AuthorsMcAllister CS,Lakhdari O,Pineton de Chambrun G,Gareau MG,Broquet A,Lee GH,Shenouda S,Eckmann L,Kagnoff MF

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