| 产品介绍 | | |
| Mouse anti Human p53 antibody, clone DO-1recognizes the human p53 tumor suppressor protein, also known as cellular tumor antigen p53 or NY-CO-13. Clone DO-1 binds to both wild type and mutant forms of the p53 protein found in various malignancies (Kernet al.1992). p53 is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells (Hauptet al.2003) and thus functions as a tumor suppressor that is involved in preventing cancer. Mutations in the p53 gene are found in about half the cases of human cancer (Joerger and Fersht 2007)Mouse anti Human p53 antibody, clone DO-1 recognizes an epitope at the N-terminal end of p53 between amino acids 20-25,common to isoforms 1-3 of p53. |
| 产品详情 | | |
Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Mouse | x |
| Rat | x |
| Bovine | √ |
| Cat | √ |
| Horse | √ |
| Green Monkey | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG conjugated to Biotin - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide 1% Bovine Serum Albumin |
| Immunogen | Recombinant human p53. |
| Approx. Protein Concentrations | IgG concentration 0.1 mg/ml |
| Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Immunohistology - Paraffin | √ | | Neat |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 1x106cells in 100ul.
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| 文献 |
| 1. Sironi, G.et al.(1999) p53 protein expression in conjunctival squamous cell carcinomas of domestic animals.Vet Ophthalmol. 2 (4): 227-231.2. Vojtěsek Bet al.(1992) An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53.J Immunol Methods. 151 (1-2): 237-44.3. Levesque, M.A.et al.(1995) Time-resolved immunofluorometric assay of p53 protein.Clin Chem. 41 (12 Pt 1): 1720-9.4. Carvalho, T.et al.(2009) Immunohistochemical evaluation of vascular urinary bladder tumors from cows with enzootic hematuria.Vet Pathol. 46 (2): 211-21.5. Phillips, A.et al.(2010) HDMX-L is expressed from a functional p53-responsive promoter in the first intron of the HDMX gene and participates in an autoregulatory feedback loop to control p53 activity.J Biol Chem. 285 (38): 29111-27.6. Bergman, L.M.et al.(2009) CtBPs promote cell survival through the maintenance of mitotic fidelity.Mol Cell Biol. 29: 4539-51.7. Hietanen, S.et al.(2000) Activation of p53 in cervical carcinoma cells by small molecules.Proc Natl Acad Sci U S A. 97 (15): 8501-6.8. Phelps, M.et al.(2003) p53-independent activation of the hdm2-P2 promoter through multiple transcription factor response elements results in elevated hdm2 expression in estrogen receptor alpha-positive breast cancer cells.Cancer Res. 63: 2616-23. |
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