| 产品介绍 | | |
| Rabbit anti Human collagen IV antibodyrecogizes human collagen IV, also known as Collagen alpha-1(IV) chain. Collagen IV is a 1497 amino acid structural extracellular matrix protein and key component of the glomerular basement membrane. Arresten, comprising the c-terminal 225 amino acids of collagen IV demonstrates potent anti tumorigenic and anti angiogenic proterties (Nyberget al.2008).Rabbit anti Human collagen IV antibody exhibits the following reactivities with Collagen IV and related proteins:Human plasma proteins do not interfere with collagen binding.Defects in the COL4A1 gene encoding collagen 4 can lead to the presentation of a variety of conditions including Brain small vessel disease with or without ocular anomalies (BSVD), demonstrating weakened brain blood vessels and retinal arteriolar tortuosity (Shahet al.2010). Hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC), characterized by large renal cysts and hematuria (Plaisieret al.2010). Porencephaly 1 (POREN1), a neurologic disease characterized by fluid filled cysts within the cerebral hemispheres, visual defect and facial paresis (Gouldet al.2005). Schizencephaly (SCHZC) presenting with full thickness cleft within the cereral hemispheres, some of the hemispheres may also be absent, replaced by cerebrospinal fluid (Yonedaet al.2013). Other conditions resulting from mutations in the COL4A1 gene also exist presenting with some of the features described for the above conditions. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - lyophilized |
Preparation | Purified IgG prepared by affinity chromatography on immobilized human collagen type IV |
Buffer Solution | 0.1M Sodium Chloride 0.01M Sodium Phosphate 0.01M Sodium Borate |
Preservative Stabilisers | 1% Mannitol1% Dextran |
Reconstitution | Use 500 µl sterile distilled water. |
Immunogen | Collagen type IV from human placenta. |
Regulatory | For research purposes only |
Guarantee | Guaranteed until date of expiry. Please see product label. |
| 存储条件 | | |
| Store at -20oC only.Storage in frost-free freezers is not recommended.Avoid repeated freezing and thawing as this may denature the antibody. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/1.5K | 1/6K |
Immunofluorescence | √ | 1/10 | 1/50 |
| Immunohistology - Paraffin1 | √ | 1/10 | 1/50 |
Immunohistology - Frozen | √ | 1/10 | 1/50 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Pretreatment is required with 1 mg/ml pepsin in 0.5 M acetic acid for two hours at 37ºC and multiple buffer washes.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls. |
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| 文献 |
| 1. Jones. E.A.V.et al. (2008) Separating genetic and hemodynamic defects in neuropilin 1 knockout embryos.Development. 135: 2479-88.2. Cunningham, K.et al.(2011) Conditional deletion of Ccm2 causes hemorrhage in the adult brain: a mouse model of human cerebral cavernous malformations.Hum Mol Genet. 20: 3198-206.3. Reis, M.et al.(2012) Endothelial Wnt/β-catenin signaling inhibits glioma angiogenesis and normalizes tumor blood vessels by inducing PDGF-B expression.J Exp Med. 209: 1611-27.4. Powner, M.B.et al.(2010) Perifoveal müller cell depletion in a case of macular telangiectasia type 2.Ophthalmology. 117: 2407-16.5. Dunér, P.et al.(2015) Increased aldehyde-modification of collagen type IV in symptomatic plaques - A possible cause of endothelial dysfunction.Atherosclerosis. 240 (1): 26-32.6. Powner, M.B.et al.(2016) Evaluation of Nonperfused Retinal Vessels in Ischemic Retinopathy.Invest Ophthalmol Vis Sci. 57 (11): 5031-7. |
QQ:1749072012