| 产品介绍 | | |
| Mouse anti Ovine Interleukin-8 antibody, clone 8M6recognizes ovine interleukin-8 (IL-8), also known as C-X-C motif chemokine 8. IL-8 is a 79 amino acid ~9-11 kDa chemoattractant for neutrophils, basophils and T-cells. IL-8 is produced by several cell types including neutrophils, monocytes and macrophages in response to inflammatory stimulation. Mouse anti ovine Interleukin-8 antibody, clone 8M6 shows no cross-reactivity with ovine IL-1 beta, IL-6, MCP-1 or TNF alpha.Responses to infectious stimuli may vary among ovine species, the response toMannheimia haemolytica, a causative agent of pneumonia, peritonitis and gangrenous mastitis in ovids, is exaggerated in Bighorn sheep (Ovis canadensis) compared to domestic sheep (Ovis aries) with significantly elevated IL-8 levels in response to infection (Herndonet al.2010).Mouse anti ovine interleukin-8 antibody, clone 8M6 has been utilized to identify cells and cell types expressing IL-8 in inflamed porcine tissue (Laursenet al.2014) showing here also that neutrophils are the predominant cell type expressing IL-8 whilst epithelial and endothelial cells in the vicinity of inflammatory lesions also express the cytokine. Clone 8M6 neutralizes the bioactivity of ovine IL-8.Mouse anti Ovine Interleukin-8 antibody, clone 8M6 has been used in conjunction with Rabbit anti Sheep Interleukin-8 antibody (AHP425) for the development of a sensitive ELISA to measure IL-8 concentrations in bovine samples (Croninet al.2015). |
| 产品详情 | | |
Target Species | Sheep |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Monkey | √ |
| Dog | √ |
| Rabbit | √ |
| Bovine | √ |
| Pig | √ |
| Mustelid | √ |
| Ferret | √ |
| Mink | √ |
| Cat | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
| Immunogen | Recombinant ovine IL-8. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry 1 | √ | | 1/10 |
| ELISA | √ | | 5ug/ml |
| Western Blotting | √ | | |
| Functional Assays2 | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm&trade: (Product CodeBUF09A) for this purpose.2Removal of the preservative is recommended prior to use in functional assays.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 1x106cells in 100ul.
- ELISAMouse anti Sheep Interleukin-8 antibody, clone 8M6 may be used in combination withAHP425in sandwich ELISA assays for ovine IL-8.
|
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG2a Negative Control | MCA929 | Flow | 100 Tests |
| 文献 |
| 1. Rainard, P.et al.(2008) The chemokine CXCL3 is responsible for the constitutive chemotactic activity of bovine milk for neutrophils.Mol Immunol. 45 (15): 4020-7.2. Stinson LFet al.(2014) Effects of cytokine-suppressive anti-inflammatory drugs on inflammatory activation inex vivohuman and ovine fetal membranes.Reproduction. 147 (3): 313-20.3. Tahoun, A.et al.(2015) Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella.Vet Res. 46: 9.4. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.Vet Immunol Immunopathol. 132 (2-4): 109-15.5. Erneholm, K.et al.(2016) Genital tract lesions in sexually mature Göttingen minipigs during the initial stages of experimental vaginal infection withChlamydia trachomatisserovar D.BMC Vet Res. 12 (1): 200.6. Melville, J.M.et al.(2017) Human amnion epithelial cells modulate the inflammatory response to ventilation in preterm lambs.PLoS One. 12 (3): e0173572.7. Magiri, R.et al.(2019) Innate immune response profiles in pigs injected with vaccine adjuvants polydi(sodium carboxylatoethylphenoxy)phosphazene (PCEP) and Emulsigen.Vet Immunol Immunopathol. 209: 7-16. |
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