Rat anti human TNF alpha:Preservative Free
货号：MCA1560XZ
规格：0.5 mg
价格：6401
产品类型：流式抗体
品牌：Bio-Rad
抗原：TNF alpha
物种：人
宿主：大鼠
抗体亚型：IgG1
克隆号：MP9-20A4
荧光染料：Preservative Free
| 产品介绍 | | |
| Rat anti Human TNF alpha antibody, clone MP9-20A4recognizes human tumor Necrosis Factor - alpha (TNF alpha), also known as TNFSF2. This ~17kDa cytokine is expressed by activated macrophages, monocytes, neutrophils, T cells and NK cells.Rat anti Human TNF alpha antibody, clone MP9-20A4 is reported to act as a blocking antibody (Abramset al.2001). |
| 产品详情 | | |
Target Species | Human |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | None present |
| Carrier Free | Yes |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| Store at -20°C only.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend micro-centrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry 1 | √ | | |
| ELISA | √ | 5ug/ml | 10ug/ml |
| Immunohistology - Frozen | √ | | |
| Western Blotting | X | | |
| Functional Assays | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Membrane permeabilization is required for this application. The use of Leucoperm (Product CodeBUF09) is recommended for this purpose. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Rat IgG1 Negative Control | MCA6004GA | Flow | 0.1 mg |
| 数据 |
| Figure A. Unstimulated cells were stained with FITC conjugated Mouse anti Human CD3 (MCA463F) and purified Rat anti Human TNF alpha (MCA1560) detected with PE conjugated Goat anti Rat (STAR73). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 6 hours were stained with FITC conjugated Mouse anti Human CD3 (MCA463F) and purified Rat IgG1 (MCA6004) detected with PE conjugated Goat anti Rat (STAR73). Figure C. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 6 hours were stained with FITC conjugated Mouse anti Human CD3 (MCA463F) and purified Rat anti Human TNF alpha (MCA1560) detected with PE conjugated Goat anti Rat (STAR73). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer |
| 文献 |
| 1. Butler, D.M.et al.(1997) DBA/1 mice expressing the human TNF-alpha transgene develop a severe, erosive arthritis: characterization of the cytokine cascade and cellular composition.J Immunol. 159 (6): 2867-76.2. Vujanovic, L.et al.(2010) Virally infected and matured human dendritic cells activate natural killer cells via cooperative activity of plasma membrane-bound TNF and IL-15.Blood. 116: 575-83.3. Abrams, J.S. (2001) Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies.Curr Protoc Immunol. Chapter 6: Unit 6.20.4. Evans HGet al.(2009)In vivoactivated monocytes from the site of inflammation in humans specifically promote Th17 responses.Proc Natl Acad Sci U S A. 106 (15): 6232-7.5. Kobawala TPet al.(2016) Significance of TNF-α and the Adhesion Molecules: L-Selectin and VCAM-1 in Papillary Thyroid Carcinoma.J Thyroid Res. 2016: 8143695.6. Mann, E.R.et al.(2016) Compartment-specific immunity in the human gut: properties and functions of dendritic cells in the colon versus the ileum.Gut. 65 (2): 256-70.7. Kumagai, M.et al.(2012) Effective transgene expression without toxicity by intraperitoneal administration of PEG-detachable polyplex micelles in mice with peritoneal dissemination.J Control Release. 160 (3): 542-51.8. Poussin Cet al.(2014) Mechanism of an indirect effect of aqueous cigarette smoke extract on the adhesion of monocytic cells to endothelial cells in anin vitroassay revealed by transcriptomics analysis.Toxicol In Vitro. 28 (5): 896-908. |
技术参数 产品应用 IHC;ELISA;Flow；FN
产品类型 Monoclonal Antibody
推荐用于 IHC;ELISA;Flow；FN
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