| 产品介绍 | | |
| Mouse anti Rat CD71 antibody, clone OX-26recognizes rat CD71, also known as transferrin receptor, a homodimeric type II transmembrane protein, expressed by all proliferating cells and cells with a requirement for iron, including reticulocytes and capillary endothelium in brain. Clone 0X-26 also binds to a number of non-dividing normal tissues.The balance between a sufficient amount of iron uptake and prevention of accumulation of excess iron within a cell, is vitally important to maintain cellular functions such as oxygen and electron transport and mitochondrial energy metabolism, whilst preventing permanent cell and tissue damage. Transferrin receptor (CD71), transferrin and ferritin have been identified as specialised proteins which control the uptake, transport and storage of free iron in tissues, thereby maintaining iron homeostasis (Crihtonet al.1992).An imbalance in iron homeostasis within the brain has been linked with the neurodegenerative diseases, Alzheimer’s, Parkinson’s, Huntington’s and Multiple Sclerosis (Benarroch 2009).Mouse anti rat CD71 clone OX-26 is reported as suitable for use in immunoelectron microscopy (Lipardiet al.2002). OX-26 detects a band of ~95kDa in Western blotting under reducing conditions and ~195 kDa under non-reducing conditions reflecting it's homodimeric structure. |
| 产品详情 | | |
Target Species | Rat |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Reconstitution | Reconstitute with 1 ml distilled water |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose |
| Immunogen | PHA activated rat lymphocytes. |
| Fusion Partners | Spleen cells from immunised Balb/c mice were fused with cells from the NS1 mouse myeloma cell line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul. |
| 数据 |
| Figure A. Alexa Fluor 700 conjugated Mouse anti Rat CD45 (MCA43A700) and RPE conjugated Mouse IgG2a isotype control (MCA1210PE). Figure B. Alexa Fluor 700 conjugated Mouse anti Rat CD45 (MCA43A700) and RPE conjugated Mouse anti Rat CD71 (MCA155PE). All experiments performed on red cell lysed rat bone marrow gated on live, single cells in the presence of 10% rat serum. Data acquired on the ZE5™ Cell Analyzer |
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG2a Negative Control:RPE | MCA1210PE | Flow | 100 Tests |
| 文献 |
| 1.Moos, T.et al.(2003) Delivery of transferrin and immunoglobulins to the ventricular system of the rat.Front Biosci. 8: a102-9.2. Fabriek, B.O.et al.(2007) The macrophage CD163 surface glycoprotein is an erythroblast adhesion receptor.Blood. 109: 5223-9.3. Rathnasamy, G. et al.et al.(2011) Iron and Iron Regulatory Proteins in Amoeboid Microglial Cells Are Linked to Oligodendrocyte Death in Hypoxic Neonatal Rat Periventricular White Matter through Production of Proinflammatory Cytokines and Reactive Oxygen/Nitrogen SpeciesJ. Neurosci 31: 17982-95.4. Loureiro, J.A.et al.(2016) Cellular uptake of PLGA nanoparticles targeted with anti-amyloid and anti-transferrin receptor antibodies for Alzheimer's disease treatmentColloids and Surfaces B: Biointerfaces Apr 20 [Epub ahead of print]5. Loureiro, J.A.et al.(2015) Dual ligand immunoliposomes for drug delivery to the brain.Colloids Surf B Biointerfaces. 134: 213-9.6. Picard, E.et al.(2015) Targeting iron-mediated retinal degeneration by local delivery of transferrin.Free Radic Biol Med. 89: 1105-21.7. Ashrafzadeh, M.S.et al.(2020)In vivoGlioblastoma Therapy Using Targeted Liposomal Cisplatin.Int J Nanomedicine. 15: 7035-49. |
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