SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), PE, eBioscience™

货号：12-8843-42,12-8843-41

规格：100 tests,25 tests

价格：2580,1649

产品类型：流式抗体

品牌：eBioscience

物种：人/小鼠

宿主：小鼠

抗体亚型：其它

荧光染料：PE

类型:	单抗	同型对照：	IgG3
免疫原性：		用法：	5 µL (0.06 µg)/test(Flow)

Product Specific InformationDescription: The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein. Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), human induced pluripotent stem (iPS) cells, and human embryonic stem cells (ES) with a decrease in expression upon differentiation. Recently expression has also been found on bone marrow mesenchymal stem cells.The eBioMC-813-70 antibody has been reported to crossreact to mouse, rabbit, canine, chicken, and Old and New World monkeys.Applications Reported: This eBioMC-813-70 (MC-813-70) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioMC-813-70 (MC-813-70) antibody has been pre-titrated and tested by flow cytometric analysis of the 2102Ep cell line. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationSSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

SSEA4 Antibody (12-8843-42) in FlowStaining of the 2102Ep cell line with Mouse IgG3 Isotype Control PE (Product # 12-4742-42) (open histogram) or Anti-Human SSEA-4 PE (filled histogram). Total viable cells were used for analysis.

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参考文献：

1.Stem cells and developmentSimplified Footprint-Free Cas9/CRISPR Editing of Cardiac-Associated Genes in Human Pluripotent Stem Cells.2.Scientific reportsContribution and Mobilization of Mesenchymal Stem Cells in a mouse model of carbon tetrachloride-induced liver fibrosis."12-8843 was used in Flow cytometry/Cell sorting to provide further evidence to show the role of BM-MSCs in liver fibrosis and to elucidate the mechanism underlying MSCs mobilization in our early liver fibrosis mice model induced by CCl4."AuthorsLiu Y,Yang X,Jing Y,Zhang S,Zong C,Jiang J,Sun K,Li R,Gao L,Zhao X,Wu D,Shi Y,Han Z,Wei L3.Scientific reportsContribution and Mobilization of Mesenchymal Stem Cells in a mouse model of carbon tetrachloride-induced liver fibrosis."12-8843 was used in Flow cytometry/Cell sorting to provide further evidence to show the role of BM-MSCs in liver fibrosis and to elucidate the mechanism underlying MSCs mobilization in our early liver fibrosis mice model induced by CCl4."AuthorsLiu Y,Yang X,Jing Y,Zhang S,Zong C,Jiang J,Sun K,Li R,Gao L,Zhao X,Wu D,Shi Y,Han Z,Wei L

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