| 产品介绍 | | |
| Mouse anti Human CD104 antibody, clone 450-9Drecognizes the human beta4 integrin, also known as CD104. CD104 is a ~205 kDa glycoprotein which associates with the alpha6 integrin to form the alpha6/beta4 complex. CD104 is expressed on epithelial cells, Schwann cells and various tumor cell lines.Mouse anti Human CD104 antibody, clone 450-9D recognizes an extracellular epitope on the CD104 molecule. |
| 产品详情 | | |
Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Mustelid | Expected from Sequence |
| Ferret | √ |
| Mink | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09%Sodium Azide |
| Carrier Free | Yes |
| Immunogen | Purified alpha6 beta4 integrin from A431 cells. |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow | √ | 1/25 | 1/100 |
| Immunofluorescence | √ | | |
| Immunohistology - Frozen1 | √ | 1/500 | 1/1000 |
| Immunohistology - Paraffin | x | | |
| Immunoprecipitation | √ | | |
| Western Blotting | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
- Histology Positive Control TissueHuman Tonsil
|
| 数据 |
| Staining of human tonsil cryostat section with Mouse anti human CD104 antibody, clone 450-9D (MCA1456). |
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control | MCA928 | Flow | 100 Tests |
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| 文献 |
1. Kennel, S.J.et al.(1990) Second generation monoclonal antibodies to the human integrin alpha 6 beta 4.Hybridoma. 9 (3): 243-55.2. Barbaro, V.et al.(2009) Reconstruction of a human hemicornea through natural scaffolds compatible with the growth of corneal epithelial stem cells and stromal keratocytes.Mol Vis. 15: 2084-93.3. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.Vet Immunol Immunopathol. 132:109-15.4. Smith, K.et al.(2011) Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action.Immunology. 132: 256-65.5. Alam, H.et al.(2011) Loss of keratins 8 and 18 leads to alterations in α6β4-integrin-mediated signalling and decreased neoplastic progression in an oral-tumour-derived cell line.J Cell Sci. 124: 2096-106.6. Lai-cheong, J.E.et al.(2009) Loss-of-function FERMT1 mutations in kindler syndrome implicate a role for fermitin family homolog-1 in integrin activation.Am J Pathol. 175 (4): 1431-41.7. Groves, R.W.et al.(2010) A homozygous nonsense mutation within the dystonin gene coding for the coiled-coil domain of the epithelial isoform of BPAG1 underlies a new subtype of autosomal recessive epidermolysis bullosa simplex.J Invest Dermatol. 130 (6): 1551-7.8. Aasted, B. & Viuff, B. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.Vet Immunol Immunopathol. 119 (1-2): 27-37.
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