Rat anti mouse CD19:PE-Cy5
货号：MCA1439C
规格：100 Tests/1ml
价格：5126
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD19
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a
克隆号：6D5
荧光染料：PE-Cy5
| 产品介绍 | | |
| Rat anti Mouse CD19 antibody, clone 6D5recognizes the murine CD19 cell surface antigen, a ~95 kDa glycoprotein expressed by B lymphocytes. Rat anti Mouse CD19 antibody, clone 6D5 recognizes the same, or a closely related epitope as clone Rat anti Mouse CD19 antibody, clone ID3 in cross-competition assays. |
| 产品详情 | | |
Target Species | Mouse |
| Product Form | Purified IgG conjugated to R. Phycoerythrin - Cy5 (RPE-Cy5) - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3) Stabilizing agent (sucrose) |
| Immunogen | Human K562 cell line transfected with murine CD19. |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
Fusion Partners | Spleen cells from immunised rats were fused with cells of the P3X63.Ag8.653 myeloma cell line. |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
RPE-Cy5 488nm laser | 496 | 667 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| Acknowledgements | Cy® and CyDye® are registered trademarks of GE Healthcare |
| 存储条件 | | |
| Store at +4oC.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | Neat | 1/5 |
| 相关试剂 |
| Description | Product Code | Applications | Pack Size |
| Mouse Seroblock FcR | BUF041A | Flow | 0.1mg |
| Mouse Seroblock FcR | BUF041B | Flow | 0.1mg |
| 文献 |
| 1.Starck, J.et al.(2010) Inducible Fli-1 gene deletion in adult mice modifies several myeloid lineage commitment decisions and accelerates proliferation arrest and terminal erythrocytic differentiation.Blood. 116: 4795-805.2. Scotland, R.S.et al.(2011) Sex differences in resident immune cell phenotype underlie more efficient acute inflammatory responses in female mice.Blood. 118 (22): 5918-27.3. White, H.N. and Meng, Q.H. (2012) Recruitment of a Distinct but Related Set of VH Sequences into the Murine CD21hi/CD23- Marginal Zone B Cell Repertoire to That Seen in the Class-Switched Antibody Response.J Immunol. 188: 287-93.4. Reynaud, J.M.et al.(2014) Human herpesvirus 6A infection in CD46 transgenic mice: viral persistence in the brain and increased production of proinflammatory chemokines via Toll-like receptor 9.J Virol. 88: 5421-36.5. Candolfi, M.et al.(2011) B cells are critical to T-cell-mediated antitumor immunity induced by a combined immune-stimulatory/conditionally cytotoxic therapy for glioblastoma.Neoplasia. 13: 947-60.6. Takabayashi, H.et al.(2014) Anti-inflammatory activity of bone morphogenetic protein signaling pathways in stomachs of mice.Gastroenterology. 147: 396-406.e7.7. Weiss-Gayet, M.et al.(2016) Notch Stimulates Both Self-Renewal and Lineage Plasticity in a Subset of Murine CD9High Committed Megakaryocytic Progenitors.PLoS One. 11 (4): e0153860.8. Meng, Q.H. & White, H.N. (2017) CD21intCD23+follicular B cells express antigen-specific secretory IgM mRNA as primary and memory responses.Immunology. 151 (2): 211-8.9. Mccubbrey, A.L.et al.(2016) MicroRNA-34a Negatively Regulates Efferocytosis by Tissue Macrophages in Part via SIRT1.J Immunol. 196 (3): 1366-75.10. Vila-Caballer, M.et al.(2019) Disruption of the CCL1-CCR8 axis inhibits vascular Treg recruitment and function and promotes atherosclerosis in mice.J Mol Cell Cardiol. 132: 154-63. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
推荐用于 Flow
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