FITC (5/6-fluorescein isothiocyanate), mixed isomer
货号：46425,46424
规格：100 mg,1 g
价格：1326,3990
产品类型：蛋白标记
品牌：Thermo Fisher
| Fluorescein isothiocyanate (FITC) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. The isothiocyanate variety of fluorescein efficiently labels antibodies and other purified proteins at primary amines (lysine side chains) |
| 应用 |
| • Label antibodies for use as immunofluorescent probes• Label oligonucleotides for hybridization probes• Detect proteins in gels and on Western blots |
| 数据 |
| Detection of α-tubulin in A549 cells demonstrates use of fluorescein-labeled secondary antibodyCells were grown in 96-well microplates for 18-20 hrs, fixed with 4% paraformaldehyde (Part No. 28906) and permeabilized with 0.1% Surfact-Amps X-100 (Part No. 28314). Cells were then probed with a mouse anti-α-tubulin primary antibody (0.4µg/mL) and Fluorescein-goat anti-mouse secondary antibody (2µg/mL). Nuclei were labeled with Hoechst Dye. Images were acquired by fluorescence microscopy.A.Fluorescence image shows a delicate network of α-tubulin (pseudo-colored green) located exclusively in the cytoplasm.B.Nuclear counterstain with Hoechst Dye (pseudo-colored blue)C.Merged image. Chemical structures of FITC and NHS-FluoresceinBoth of these compounds allow fluorescent labeling of primary amines on proteins and other molecules. |
QQ:1749072012