mouse anti bovine CD11b:PE
货号：MCA1425PE
规格：100 Tests
价格：4446
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD11b
物种：Bovine
宿主：小鼠
抗体亚型：IgG2b
克隆号：CC126
荧光染料：PE
| 产品介绍 | | |
| Mouse anti Bovine CD11b antibody, clone CC126recognizes the bovine CD11b cell surface antigen. |
| 产品详情 | | |
Target Species | Bovine |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Goat | √ |
| Sheep | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Reconstitution | Reconstitute with 1.0 ml distilled water |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin 5% Sucrose |
| Approx. Protein Concentrations | IgG concentration 0.1 mg/ml |
Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line. |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
RPE 488nm laser | 496 | 578 |
| RPE 561nm laser | 546 | 578 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| Store at +4°C. DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | | 1/10 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 1 x106cells in 100ul. |
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG2b Negative Control:RPE | MCA691PE | F | 100 Tests |
| 文献 |
| 1. Gondaira, S.et al.(2020) Immunosuppression in Cows following Intramammary Infusion ofMycoplasma bovis.Infect Immun. 88 (3) Feb 20 [Epub ahead of print].2. Khosa, S.et al.(2020) Bovine Adenovirus-3 Tropism for Bovine Leukocyte Sub-Populations.Viruses. 12 (12) Dec 12 [Epub ahead of print].3. Ramm, R.et al.(2020) Decellularization combined with enzymatic removal of N-linked glycans and residual DNA reduces inflammatory response and improves performance of porcine xenogeneic pulmonary heart valves in an ovinein vivomodel.Xenotransplantation. 27 (2): e12571.4. Ramos, A.et al.(2018) Melatonin enhances responsiveness to Dichelobacter nodosus vaccine in sheep and increases peripheral blood CD4 T lymphocytes and IgG-expressing B lymphocytes.Vet Immunol Immunopathol. 206: 1-8.5. Okino, C.H.et al.(2020) A polymorphic CD4 epitope related to increased susceptibility toBabesia bovis.in Canchim calves.Vet Immunol Immunopathol. 230: 110132.6. Park, D.S.et al.(2021) Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress.Cell Stress Chaperones. 26 (4): 705-20.7. Radley, G.et al.(2020)In Vitro.Benchmarking Study of Ventricular Assist Devices in Current Clinical Use.J Card Fail. 26 (1): 70-79.8. Colombatti Olivieri, M.A.et al.(2021) Evaluation of a virulent strain ofMycobacterium avium.subsp.paratuberculosis.used as a heat-killed vaccine.Vaccine. NNov 10;S0264-410X(21)01433-X.9. Casaro, S.et al.(2022) Flow cytometry panels for immunophenotyping dairy cattle peripheral blood leukocytesVet Immunol Immunopathol. 248: 110417. |
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