| 产品介绍 | | |
| Rat anti Pig CD44 antibody, clone MAC329recognizes the porcine CD44 cell surface antigen which is involved in cell-cell interactions, cell adhesion and migration. CD44 is also a receptor for hyaluronic acid and can interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). |
| 产品详情 | | |
Target Species | Pig |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Dog | √ |
| Horse | √ |
| Bovine | √ |
| Goat | √ |
| Sheep | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin |
| Immunogen | Purified porcine CD44 |
Approx. Protein Concentrations | IgG concentration 0.1mg/ml |
| Fusion Partners | Spleen cells from immunised LOU Rats were fused with cells of the Y3.Ag1.2.3 myeloma cell line |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | 1/10 | 1/200 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul whole blood |
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Rat IgG1 Negative Control | MCA6004GA | Flow | 0.1 mg |
| 相关产品 |
| Description | Product Code | Applications | Pack Size |
| Mouse anti Pig CD3:FITC | MCA5951F | Flow | 0.1mg |
| 数据 |
| Figure A. FITC conjugated Mouse anti Pig CD3 (MCA5951F) and Rat IgG1 isotype control (MCA6004) detected with Goat anti Rat IgG:RPE (STAR73). Figure B. FITC conjugated Mouse anti Pig CD3 (MCA5951F) and Rat anti Pig CD44 (MCA1449GA) detected with Goat anti Rat IgG:RPE (STAR73). All experiments performed on red cell lysed porcine blood gated on lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5™ Cell Analyzer. |
| 文献 |
| 1.Yang, H.et al.(1993) Preparation and reactivities of anti-porcine CD44 monoclonal antibodies.Scand J Immunol. 37 (4): 490-8.2.Kasper, M.et al.(1996) Expression of CD44 isoforms during bleomycin-or radiation-induced pulmonary fibrosis in rats and mini-pigs.Histochem Cell Biol. 105 (3): 221-30.3.Gyongyosi, M.et al. (2010) Differential effect of ischaemic preconditioning on mobilisation and recruitment of haematopoietic and mesenchymal stem cells in porcine myocardial ischaemia-reperfusion.Thromb Haemost. 103: 1-9.4.Smith, M.M.et al.(2008) Significant synovial pathology in a meniscectomy model of osteoarthritis: modification by intra-articular hyaluronan therapy.Rheumatology (Oxford). 47:1172-8.5.Sanchez, M.A.et al.(2004) Organ-specific immunity in canine visceral leishmaniasis: analysis of symptomatic and asymptomatic dogs naturally infected with Leishmania chagasi.Am J Trop Med Hyg. 70: 618-24.6.Lacroux, C.et al.(2012) Study of canine cutaneous melanocytic tumours: evaluation of histological and immunohistochemical prognostic criteria in 65 casesRevue de Médecine Vétérinaire 163: 393-401.7.Zimmermann, C.E.et al.(2021) Characterization of porcine mesenchymal stromal cells and their proliferative and osteogenic potential in long-term culture.J Stem Cells Regen Med. 17 (2): 49-55. |
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