| 产品介绍 | | |
| Mouse anti Pig CD45 allotypic variant antibody, clone MAC323recognizes an allotypic determinant of porcine CD45, also known as the leucocyte common antigen (LCA). Mouse anti Pig CD45 allotypic variant antibody, clone MAC323 has been shown to recognize CD45 in pigs of the Large White and German Landrace strains. Another antibody, cloneK252.1E4, recognizes a monomorphic determinant on porcine CD45. When K252.1E4 is used in conjunction with MAC323, it can be shown that MAC323 stains a subset of the leukocytes that are detected by K252.1E4; indicating a loss of the epitope in MAC323-veanimals rather than loss of the CD45 antigen. Cross-breeding positive vs. negative animals indicate that the MAC323 gene is inherited in a simple Mendelian autosomal dominant pattern (Binnset al.1995). |
| 产品详情 | | |
Target Species | Pig |
| Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Carrier Free | Yes |
| Immunogen | Porcine peripheral blood lymphocytes |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | 1/25 | 1/100 |
| Immunohistology - Frozen | √ | | 1/10 |
| Immunohistology - Paraffin | | | 1/10 |
| Immunohistology - Resin1 | | | 1/10 |
| Immunoprecipitation | | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment of resin sections. This product does not require antigen retrieval prior to staining paraffin sections.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG2a Negative Control | MCA929 | Flow | 100 Tests |
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse anti Pig CD3:FITC | MCA5951F | Flow | 0.1mg |
| 数据 |
| Figure A. FITC conjugated mouse anti porcine CD3 (MCA5951F) and purified mouse IgG2a isotype control (MCA929) detected with RPE conjugated goat anti mouse IgG (STAR133PE). Figure B. FITC conjugated mouse anti porcine CD3 (MCA5951F) and purified mouse anti porcine CD45 (MCA1447) detected with RPE conjugated goat anti mouse IgG (STAR133PE). All experiments performed on red cell lysed porcine blood gated on lymphocytes. Data acquired on the ZE5™ Cell Analyzer. |
| 文献 |
| 1. Binns, R.M.et al. (1995) Genetically determined CD45 variant of value in leucocyte tracingin vivoin the pig.Immunology 86: 25-33.2. Goodchild, T.et al.(2006) Safety of intramyocardial injection of autologous bone marrow cells to treat myocardial ischemia in pigs.Cardiovasc Revasc Med. 7: 136-45.3. Anttila, A.et al.(2003) T-cell-mediated mucosal immunity is attenuated in experimental necrotizing enterocolitis.Pediatr Surg Int. 19: 326-30.4. Richter, Y.et al.(2004) Dynamic flow alterations dictate leukocyte adhesion and response to endovascular interventions.J Clin Invest. 113: 1607-14.5. Kozian, A.et al.(2010) Increased alveolar damage after mechanical ventilation in a porcine model of thoracic surgery.J Cardiothorac Vasc Anesth. 24 (4): 617-23.6. Nugent, H.M.et al.(2012) Ultrasound-guided percutaneous delivery of tissue-engineered endothelial cells to the adventitia of stented arteries controls the response to vascular injury in a porcine model.J Vasc Surg. 56 (4): 1078-88.7. van Ditzhuijzen, N.S.et al.(2017) Neoatherosclerosis development following bioresorbable vascular scaffold implantation in diabetic and non-diabetic swine.PLoS One. 12 (9): e0183419. |
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