Thyroid Stimulating Hormone Receptor antibody
货号：MCA1281
规格：0.2 mg
价格：3783
产品类型：流式抗体
品牌：Bio-Rad
物种：人
宿主：小鼠
抗体亚型：IgG1
克隆号：2C11
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti Human thyroid stimulating hormone receptor antibody, clone 2C11recognizes the human thyroid stimulating hormone receptor (TSHR) otherwise known as the thyrotropin receptor. TSHR is an important molecule in controlling the growth and function of the normal thyroid.Mouse anti Human thyroid stimulating receptor antibody recognises both native and denatured TSH receptor (binding to an epitope at the carboxy terminus between amino acids 354 and 359). It does inhibit binding of TSH. No cross reactivity has been observed with related LH and FSH receptors.Mouse anti Human thyroid stimulating hormone receptor antibody, clone 2C11 recognizes the mutant TSH receptor known as I167N as well as the wild type molecule (Costagliolaet al.1998) |
| 产品详情 | | |
Target Species | Human |
| Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Immunogen | Recombinant Human TSH receptor. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the NS1/Ag4.1 mouse myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | | |
| ELISA | √ | | |
| Immunohistology - Frozen | X | | |
| Immunoprecipitation | √ | 5ug/ml | 10ug/ml |
| Western Blotting | √ | 5ug/ml | 10ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 同型 |
| Description | Product Code | Applications | Pack Size |
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
| 相关产品 |
| Description | Product Code | Applications | Pack Size |
| Human Seroblock | BUF070A | F | 50 Test |
| Human Seroblock | BUF070B | F | 200 Test |
| 文献 |
| 1. Neumann, S.et al.(2011) A new small-molecule antagonist inhibits Graves' disease antibody activation of the TSH receptor.J Clin Endocrinol Metab. 96: 548-54.2. Narumi Set al.(2011) Nonclassic TSH resistance: TSHR mutation carriers with discrepantly high thyroidal iodine uptake.J Clin Endocrinol Metab. 96 (8): E1340-5.3. Allen, M.D.et al.(2011) Occupancy of both sites on the thyrotropin (TSH) receptor dimer is necessary for phosphoinositide signaling.FASEB J. 25: 3687-94.4. Chen, C.R.et al.(2001) A full biological response to autoantibodies in Graves' disease requires a disulfide-bonded loop in the thyrotropin receptor N terminus homologous to a laminin epidermal growth factor-like domain.J Biol Chem. 276 (18): 14767-72.5. Read, M.L.et al.(2011) Proto-oncogene PBF/PTTG1IP regulates thyroid cell growth and represses radioiodide treatment.Cancer Res. 71 (19): 6153-64.6. Krause, K.et al.(2012) Comparative proteomic analysis to dissect differences in signal transduction in activating TSH receptor mutations in the thyroid.Int J Biochem Cell Biol. 44 (2): 290-301.7. Claus, M.et al.(2005) A hydrophobic cluster in the center of the third extracellular loop is important for thyrotropin receptor signaling.Endocrinology. 146 (12): 5197-203. |
技术参数 产品应用 Flow;IHC;ELISA;IP；WB
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-33596.htm
QQ:1749072012