| 产品介绍 | | |
| Mouse anti Human CD88 antibody, clone S5/1recognizes the C5a receptor (C5aR) CD88, which is predominantly expressed on cells of the myeloid lineage. Clone S5/1 was raised against a synthetic peptide comprising the N-terminal extracellular domain of the C5aR (met1-Asn31) and has recently been shown to recognise the heptameric peptide (D15DKDTLD21).Clone S5/1 has been shown to inhibit the binding of C5a to its receptor. |
| 产品详情 | | |
Target Species | Human |
Species Cross-Reactivity | Target Species | Cross Reactivity |
| Rabbit | √ |
| Mustelid | Expected from Sequence |
| Bovine | √ |
| Ferret | √ |
| Mink | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Reconstitution | Reconstitute with 1.0 ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. |
Preparation | Purified IgG prepared by affinity chromatography on Protein A |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin 5% Sucrose |
| Immunogen | C5aR - peptide: Met1 - Asn31. |
Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8 myeloma cell line. |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at +4oC.After reconstitution store at +4oC.DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 1x106cells in 100ul |
| 数据 |
| Mouse anti Human CD88 antibody, clone S5/1(MCA1283) used to stain formalin fixed, paraffin embedded human spleen sections followed by biotinylated anti Mouse IgG and streptavidin alkaline phosphatase (red). Nuclei were counterstained with hematoxylin (blue). |
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| 文献 |
| 1. Schreiber, A.et al.(2009) C5a receptor mediates neutrophil activation and ANCA-induced glomerulonephritis.J Am Soc Nephrol. 20: 289-98.2. Eglite, S.et al.(2000) Requirements for C5a receptor-mediated IL-4 and IL-13 production and leukotriene C4 generation in human basophils.J Immunol. 165: 2183-9.3. Conroy, A.et al.(2009) C5a enhances dysregulated inflammatory and angiogenic responses to malaria in vitro: potential implications for placental malaria.PLoS One. 4: e4953.4. Kraft, K.et al.(2001) Characterization of sequence determinants within the carboxyl-terminal domain of chemokine receptor CCR5 that regulate signaling and receptor internalization.J Biol Chem. 276: 34408-18.5. Fukuoka, Y.et al.(2008) Generation of anaphylatoxins by human beta-tryptase from C3, C4, and C5.J Immunol. 180: 6307-16.6. Bettoni, S.et al.(2017) Interaction between Multimeric von Willebrand Factor and Complement: A Fresh Look to the Pathophysiology of Microvascular Thrombosis.J Immunol. 199 (3): 1021-40.7. Huang, L.et al.(2005) Discovery of human antibodies against the C5aR target using phage display technology.J Mol Recognit.18: 327-33.8. Tseng CWet al.(2015) Increased Susceptibility of Humanized NSG Mice to Panton-Valentine Leukocidin andStaphylococcus aureusSkin Infection.PLoS Pathog. 11 (11): e1005292. |
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