| 产品介绍 | | |
| Mouse anti Human CD39, clone A1recognizes the human CD39 cell surface antigen, a ~70-100 kDa molecule expressed on peripheral blood B cells, T cells and monocytes, and weakly expressed by granulocytes.CD39 has intrinsic ecto-ATPase activity (Wanget al.1996), and expression can be induced on T cells and increased on B cells, as a late activation antigen (Maliszewskiet al.1994).Mouse anti Human CD39, clone A1 has been shown to block MHC independent target cell recognition by hapten-specific CTL (Stocklet al.2001). |
| 产品详情 | | |
Target Species | Human |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Reconstitution | Reconstitute with 1 ml distilled water |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose |
| Immunogen | PHA activated human lymphocytes |
| Approx. Protein Concentrations | IgG concentration 0.1 mg/ml |
Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
RPE 488nm laser | 496 | 578 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.This product should be stored undiluted.DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | | Neat |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Flow CytometryUseUse 10ul of the suggested working dilution to label 106cells or 100ul whole blood
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| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
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| 文献 |
| 1. Stöckl, J.et al.(2001) Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL.J Immunol. 167 (5): 2724-33.2. Scholzen, A.et al.(2009) Plasmodium falciparum-mediated induction of human CD25Foxp3 CD4 T cells is independent of direct TCR stimulation and requires IL-2, IL-10 and TGFbeta.PLoS Pathog. 5: e1000543.3. Borsellino, G.et al.(2007) Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppressionBlood. 110:1225-32.4. Mittag, D.et al.(2010) The effector T cell response to ryegrass pollen is counterregulated by simultaneous induction of regulatory T cells.J Immunol. 184: 4708-16.5. Loeuillet, C.et al.(2008) In vitro whole-genome analysis identifies a susceptibility locus for HIV-1.PLoS Biol. 6: e32.6. Rawstron, A.C.et al.(2010) Chronic lymphocytic leukaemia (CLL) and CLL-type monoclonal B-cell lymphocytosis (MBL) show differential expression of molecules involved in lymphoid tissue homing.Cytometry B Clin Cytom. 78 Suppl 1: S42-6.7. Alam, M.S.et al.(2009) CD73 is expressed by human regulatory T helper cells and suppresses proinflammatory cytokine production and Helicobacter felis-induced gastritis in mice.J Infect Dis. 199: 494-504.8. Moreno-Fernandez, M.E.et al.(2011) Regulatory T cells control HIV replication in activated T cells through a cAMP-dependent mechanism.Blood. 117: 5372-80.9. Guevara-Flores, A.et al.(2008) 5'-p-Fluorosulfonyl benzoyl adenosine inhibits an ecto-ATP-diphosphohydrolase in the tegument surface of Taenia crassiceps cysticerci.Mol Biochem Parasitol. 162: 123-33.10.Glenn, J.R.et al.(2008) Raised levels of CD39 in leucocytosis result in marked inhibition of ADP-induced platelet aggregation via rapid ADP hydrolysis.Platelets. 19: 59-69.11. Häusler SFet al.(2014) Anti-CD39 and anti-CD73 antibodies A1 and 7G2 improve targeted therapy in ovarian cancer by blocking adenosine-dependent immune evasion.Am J Transl Res. 6 (2): 129-39. |
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