SOX2 Monoclonal Antibody (20G5)

货号： MA1-014

规格：100 µg

价格：4070

产品类型：流式抗体

品牌：Thermo Fisher

抗原：SOX2

物种：人

宿主：小鼠

抗体亚型：其它

克隆号：20G5

荧光染料：其它

类型:	单抗	同型对照：	IgG1, kappa
免疫原性：	Full-length human recombinant protein expressed in bacteria	用法：	1-3 µL(ChIP);1:100(Flow);1:200(ICC);1:200(IF);5 µg(IP);1:1000 - 1:2000(WB)

Product Specific InformationWestern blot analysis of MA1-014 specifically detects SOX2 protein at ~36 kDa in the nucleus of human and mouse embryonal carcinoma and iPS cells.Target InformationSOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

SOX2 Antibody (MA1-014) in IFImmunofluorescent analysis of Sox2 (green) in human neural stem cells derived from PD-3 iPSCs using Gibco® PSC Neural Induction Medium (Product # A1647801). The cells were fixed and permeabilized using Image-IT® Fixation/Permeabilization kit (Product # R37602), and blocked with blocking buffer included the kit for one hour at room temperature. Cells were stained with a Sox2 monoclonal antibody (Product # MA1-014) at a dilution of 1:200 in blocking buffer for 3 hours at room temperature, and then incubated with an DyLight 488- conjugated goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 1 hour at room temperature. Image was taken on an EVOS® FLoid® Cell Imaging Station at 10X magnification. SOX2 Antibody (MA1-014) in IFImmunofluorescent analysis of Sox2 (green) in H9 embryonic stem cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a Sox2 monoclonal antibody (Product # MA1-014) at a dilution of 1:200 overnight at 4°C, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification. SOX2 Antibody (MA1-014) in IHC (P)Immunohistochemistry was performed on deparaffinized canine fetal brain tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 20 minutes at 95°C. Following antigen retrieval tissues were blocked with Avidin/Biotin Blocking kit (Product # 00-4303) at room temperature and then probed with a Sox2 monoclonal antibody (Product # MA1-014) at a dilution of 1:200 for one hour at room temperature. Tissues were washed extensively with TBS + 0.025% Triton X-100 (Product # 28314). Detection was performed using a goat anti-mouse HRP secondary antibody (Product # 31430) at a dilution of 1:500 followed by colorimetric detection using metal enhanced DAB (Product # 34065). Tissues were counterstained with hematoxylin and prepped for mounting. Images were taken on a Zeiss Axiovision microscope at 20X magnification. The bottom layer with a dense staining of Sox2 is in the subependymal plate, where there are large numbers of progenitor cells. The upper layer is neuroparenchyma where cells are differentiated, therefore less staining of Sox2. Note: Data courtesy of Innovators Program.

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参考文献：

1.Cell cycle (Georgetown, Tex.) Role of stress-activated OCT4A in the cell fate decisions of embryonal carcinoma cells treated with etoposide. 2.Nucleic acids research Conservative site-specific and single-copy transgenesis in human LINE-1 elements. "MA1-014 was used in western blot to discuss Phage lambda integrase as a transgenesis tool" AuthorsVijaya Chandra SH,Makhija H,Peter S,Myint Wai CM,Li J,Zhu J,Ren Z,D'Alcontres MS,Siau JW,Chee S,Ghadessy FJ,Dröge P

技术参数

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