Human Anti-Human IgG1 Fc Antibody clone AbD27686
货号：HCA285P
规格：0.1 mg
价格：2576
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：IgG1 Fc
物种：其它
宿主：人
抗体亚型：其它
克隆号：AbD27686
荧光染料：HRP
| 产品介绍 | | |
| Human Anti-Human IgG1 Fc Antibody clone AbD27686is a recombinant monovalent Fab antibody, that recognizes human IgG1 Fc, with an affinity of 76 nM. It has been shown to have a greatly reduced affinity (89-fold) for IgG from cynomolgus macaque compared to human IgG1. It also has strongly reduced binding to rhesus macaque IgG. It does not recognize other human immunoglobulin isotypes (IgG2, IgG3, IgG4, IgA, IgE, IgM) or mouse IgG2a.This antibody has been generated by Bio-Rad using the HuCAL® antibody library and CysDisplay® technology. It is sold for research use, with implicit permission for use for the detection of therapeutic antibodies from samples obtained from experimental animals. When tested by Bio-Rad in an antigen capture pharmacokinetic assay format, this antibody performs similarly to Mouse Anti-Human IgG (CH2 Domain) Antibody clone R10Z8E9 (MCA5748G) in 10% cynomolgus macaque serum. It recognizes a different epitope on human IgG1 Fc than clone R10Z8E9. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL PLATINUM®phage display library. Expressed in E. coli and purified using purified using NiNTA affinity chromatography. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. Conjugated to horseradish peroxidase (HRP) - liquid |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.01% Thiomersal |
| Immunogen | Recombinant human IgG1 kappa |
| Approx. Protein ConcePurityntrations | IgG concentration 0.1 mg/ml |
| Affinity | The monovalent intrinsic affinity of this antibody was measured as KD=76 nM by real time, label free molecular interaction analysis on immobilized hIgG1 kappa |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped frozen.When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | 1/100 | 1/1000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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技术参数 产品应用 elisa
产品类型 单克隆抗体
推荐用于 elisa
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