Human anti GAPDH antibody, clone AbD22549_hIgG1
货号：HCA272
规格：0.1 mg
价格：2023
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：GAPDH
物种：其它
宿主：人
抗体亚型：IgG1
克隆号：AbD22549_hIgG1
荧光染料：Purified
| 产品介绍 | | |
| Human anti GAPDH antibody, clone AbD22549_hIgG1recognizes human glyceraldehyde 3-phosphate dehydrogenase (GAPDH), a 335 amino acid multifunctional protein. The main function of GAPDH is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). It is a major glycolytic enzyme within the cytosol and is also involved in a number of intracellular processes, including membrane fusion, microtubule bundling, phosphotransferase, DNA replication and DNA repair. It has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death.GAPDH has been shown to bind to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and huntingtin (Burkeet al.1996).Since GAPDH is constitutively expressed in almost all tissues at high levels, this antibody is recommended for use as a loading control for western blotting. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.01% Thiomersal |
| Immunogen | Recombinant human GAPDH |
| Affinity | The monovalent intrinsic affinity of AbD22549 was measured as KD= 2 nM by real time, label free molecular interaction analysis on immobilized HSPA4 |
| Approx. Protein ConcePurityntrations | Antibody concentration 0.5 mg/ml. |
| Acknowledgements | This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Western Blotting | √ | 1/50 | 1/800 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Human IgG F(ab')2:HRP | STAR126P | E，WB | 0.5ml |
技术参数 产品应用 wb，ip
产品类型 多克隆抗体
推荐用于 wb，ip
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