Human anti trastuzumab, clone AbD18018_hIgG4_Pro
货号：HCA270
规格：0.1 mg
价格：5032
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：trastuzumab
物种：其它
宿主：人
抗体亚型：lgG4
克隆号：AbD18018_hIgG4_Pro
荧光染料：Purified
| 产品介绍 | | |
| Human anti trastuzumab, clone AbD18018_hIgG4_Pro, is a paratope specific, high affinity, anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug trastuzumab and inhibits it binding to its target. It is a fully human recombinant monoclonal antibody with IgG4 isotype. The antibody can be used to measure the levels of trastuzumab and biosimilar products in bioanalytical assays.Anti-trastuzumab antibody clone AbD18018 has been converted to human IgG4 isotype to offer assay developers increased flexibility in assay design. When used as an ELISA detection antibody, a secondary anti-human IgG4 antibody is required, which can be labeled according to the requirements of the user's preferred assay platform.The antibody can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug in preclinical research and clinical trials using patient sera. Antibody clones AbD16712 (HCA166) or AbD18141 (HCA169), both monovalent Fab format, are recommended as the capture antibody. Detection can be performed using clone AbD18018_hIgG4_Pro and an HRP conjugated anti-human IgG4 secondary antibody (MCA2098P).This clone is also available as an IgG1 isotype in both a purified unconjugated format (HCA177) and directly conjugated to HRP (HCA177P).Trastuzumab, also known as Herceptin®, is a drug used in the treatment of HER2 postive breast cancer and other HER2 over-expressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Trastuzumab binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells. |
| 产品详情 | | |
| Product Form | Human IgG4 antibody selected from the HuCAL® phage display library and expressed in a human cell line - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.01% Thiomersal |
| Immunogen | Trastuzumab |
| Affinity | The monovalent intrinsic affinity of this antibody was measured as KD=0.02 nM by real time, label-free molecular interaction analysis on immobilized trastuzumab. |
| Approx. Protein ConcePurityntrations | IgG concentration 0.5 mg/ml |
| Acknowledgements | This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Mouse anti Human IgG4:HRP | MCA2098P | E | 0.2mg |
技术参数 产品应用 elisa
产品类型 单克隆抗体
推荐用于 elisa
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